Transfer of arrayed chemical compositions

• US 20020136978A1
• Filed: 03/26/2002
• Published: 09/26/2002
• Est. Priority Date: 03/26/2001
• Status: Active Grant

• Current Assignee: Linden Technologies Incorporated
• Original Assignee: Linden Technologies Incorporated
• Inventors: Huang, Tai-Nang
• Granted Patent: US 6,855,501 B2
• Time in Patent Office: Days
• US Class Current: 430/126
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00614 Delimitation of the attachment areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Nucleotide preparations are transferred from a first substrate to a second substrate. One transfer method includes forming a patterned dry particulate deposition on a first substrate; positioning the first substrate in apposition to a second substrate; and transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate. The method can be used to form an array of nucleic acids.

Claims

1. A method comprising:

forming a patterned dry deposition on a first substrate, the dry deposition comprising particles that include a nucleotide;

positioning the first substrate in apposition to a second substrate; and

transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate.

2. The method of claim 1 wherein the patterned dry deposition comprises sections that include a first nucleotide and other sections that include a second nucleotide.

3. The method of claim 2 wherein the patterned dry deposition comprises at least a section for each of at least four nucleotides.

4. The method of claim 1 wherein the transferring comprising applying an electrical field.

5. The method of claim 4 wherein the voltage is between 500 and 50,000 V.

6. The method of claim 5 wherein the voltage is between 3000 and 12000 V.

7. The method of claim 4 wherein the electrical field is generated by a field generator that comprises a corotron or scorotron.

8. The method of claim 7 wherein the transferring comprises scanning the field generator across the first substrate.

9. The method of claim 1 wherein there is no physical contact between the first and second substrate.

10. The method of claim 9 where a gap formed between the first and second substrate is at least partially under vacuum.

11. The method of claim 1 wherein the first substrate is flexible.

12. The method of claim 1 wherein the positioning comprises aligning the first and second substrate.

13. The method of claim 1 further comprising coupling the nucleotide to the second substrate.

14. The method of claim 1 wherein the nucleotide has a 3 ′

or 5′

protecting group.

15. The method of claim 13 further comprising repeating the forming, positioning, transferring, and coupling for each register of the synthesized nucleic acids.

17. The method of claim 16 wherein the second substrate is glass.

18. The method of claim 17 wherein the first and second substrates are transparent.

20. The method of claim 19 wherein the polymer component is a nucleotide.

16. A method comprising:

forming a patterned dry deposition on a first substrate, the dry deposition comprising triboelectrically chargeable particles that include nucleotide that includes a protecting group and a phosphoramidite, wherein the particles are less than 30 μ

m in diameter and the first substrate is flexible;

positioning the first substrate in apposition to a second substrate; and

transferring at least a portion of the dry deposition from the first substrate to the second substrate to produce a patterned dry deposition of the nucleotide on the second substrate.

19. A method of providing a nucleic acid array, the method comprising:

providing a plurality of interim substrates, each interim substrate comprising a different nucleotide particles, the particles being disposed at different addresses of the interim substrate; and

sequentially, for each interim substrate of the plurality of interim substrates, transferring the nucleotide particles from the different addresses of the interim substrate to corresponding addresses on a target substrate, and coupling nucleotides to the target substrate to form a nucleic acid at each of the different addresses of the target substrate.