METHOD FOR CONTINUOUS MONITORING OF CHEMICAL SUBSTANCES IN FLUIDS
First Claim
1. - A method for continous monitoring of chemical substances in fluids characterised in that the microorganisms used are obtained by means of constant growth in an automatic chemostat.
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Abstract
For the last two decades much work has been done in developing biosensors with specific applications (industrial, biomedical and environmental among others) that can now compete with the classical analytical technologies but these apparatus that are based on the retention of microorganisms in membranes, have intrinsic limitations. The two principal innovations brought forward in respect of the present technology are;
The continual availability of microorganisms when using a chemostat which allows the monitoring of samples with elevated toxicity, discarding the microorganisms used after every reading, eliminating the membrane which physically separates the medium to be analyzed and the transducer, thus allowing the amplification of the linear range of the signal and drastically reducing the time needed to reach a steady signal.
It is presented a microbiosensor system to monitor on-line chemical substances in fluids. Two examples of the application are presented: online determination of biochemical oxygen demand (BOD) in water and on-line determination of toxicity in water.
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24 Claims
- 1. - A method for continous monitoring of chemical substances in fluids characterised in that the microorganisms used are obtained by means of constant growth in an automatic chemostat.
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