Apparatus for fluorescence detection on arrays
First Claim
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1. An apparatus for fluorescence detection, comprising:
- a light source for illuminating an area located on a solid support;
a means for collimating light emanating from said light source, said collimating means disposed to allow the light path of said light to pass between said light source and said solid support;
a means for focusing said collimated light onto said solid support, said focusing means disposed to allow the light path of said light to pass between said collimating means and said solid support;
a means for detecting a fluorescence emission, said fluorescence detecting means disposed to detect fluorescence emission emanating from said solid support;
a means for filtering said fluorescence emission, said filtering means disposed to filter said fluorescence emission onto said fluorescence detecting means.
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Abstract
The invention provides an apparatus for fluorescence detection, comprising a light source for illuminating a portion of a solid support; a means for splitting light emanating from the light source into two or more split beams of light; a means for polarizing the beams of light; a means for collimating the polarized light; a ¼-wave plate disposed between the collimating means and the solid support for converting the collimated light into polarized light; a means for focusing the polarized light onto the solid support; a means for detecting a fluorescence emission; a means for filtering the fluorescence emission; and a photodetector array.
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Citations
29 Claims
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1. An apparatus for fluorescence detection, comprising:
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a light source for illuminating an area located on a solid support;
a means for collimating light emanating from said light source, said collimating means disposed to allow the light path of said light to pass between said light source and said solid support;
a means for focusing said collimated light onto said solid support, said focusing means disposed to allow the light path of said light to pass between said collimating means and said solid support;
a means for detecting a fluorescence emission, said fluorescence detecting means disposed to detect fluorescence emission emanating from said solid support;
a means for filtering said fluorescence emission, said filtering means disposed to filter said fluorescence emission onto said fluorescence detecting means. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, 29)
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15. An apparatus for fluorescence detection, comprising:
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a light source for illuminating an area located on a solid support;
a collimator lens disposed to allow the light path of the light to pass between said light source and said solid support for collimating light emanating from said light source;
a focusing lens disposed to allow the light path of the light to pass between said collimator lens and said solid support for focusing said light onto said solid support;
a fluorescence detector disposed to detect fluorescence emission emanating from said solid support; and
a means for filtering said fluorescence emission, said filtering means disposed to filter said fluorescence emission onto said fluorescence detecting means.
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27. An apparatus for fluorescence detection, comprising:
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a light source for illuminating a portion of a solid support;
a means for splitting light emanating from said light source into two or more split beams of light, said splitting means disposed to allow the light path of said light to pass between said light source and said solid support;
a means for polarizing said beams of light, said polarizing means disposed to allow the light path of said light to pass between said splitting means and said solid support;
a means for collimating said polarized light, said collimating means disposed to allow the light path of said light to pass between said polarizing means and said solid support;
a ¼
-wave plate disposed to allow the light path of said light to pass between said collimating means and said solid support for converting said collimated light into polarized light;
a means for focusing said polarized light onto said solid support, said focusing means disposed to allow the light path of said light to pass between said ¼
-wave plate and said solid support;
a means for detecting a fluorescence emission, said fluorescence detecting means disposed to detect fluorescence emission emanating from said solid support;
a means for filtering said fluorescence emission, said filtering means disposed to filter said fluorescence emission onto said fluorescence detecting means; and
a photodetector array disposed orthogonal to said polarizing beam splitter for detecting light reflected from said solid support.
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Specification