Methods and systems for processing microfluidic samples of particle containing fluids
First Claim
1. A microfluidic device for processing a particle-containing fluid, comprising:
- an enrichment zone to prepare an enriched particle sample from the particle-containing fluid; and
a gas actuator to provide a gas pressure sufficient to move the enriched particle sample from the enrichment zone.
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Accused Products
Abstract
The present invention relates to a microfluidic system for processing a cell-containing liquid. The system includes a microfabricated substrate having an enrichment channel to prepare an enriched cell sample from the cell-containing liquid. A flow through member is in liquid communication with the enrichment zone. The flow through member substantially prevents cells of the cell-containing fluid from exiting the enrichment zone while allowing liquid of the cell-containing liquid to exit the enrichment zone. A gas actuator associated with the enrichment zone provides a gas pressure sufficient to move the enriched cell sample from the enrichment zone.
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Citations
35 Claims
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1. A microfluidic device for processing a particle-containing fluid, comprising:
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an enrichment zone to prepare an enriched particle sample from the particle-containing fluid; and
a gas actuator to provide a gas pressure sufficient to move the enriched particle sample from the enrichment zone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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19. A microfluidic device for processing a particle containing fluid, comprising:
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an enrichment zone configured to substantially separate an enriched particle sample from the particle-containing fluid;
an actuator to remove the enriched particle sample from the enrichment zone with essentially no dilution of the enriched particle sample.
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34. A microfluidic substrate for processing fluids comprising:
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an enrichment zone for preparing an enriched particle sample from a cell-containing fluid, a lysing module, coupled to the enrichment zone for receiving the enriched particle sample and releasing intracellular material from cells within the sample to thereby forming a lysed sample a microdroplet formation module for forming a first microdroplet of fluid from the lysed sample, a mixing module for mixing said first microdroplet with a second microdroplet comprising a reagent to form a third microdroplet, and an amplification module for amplifying intercellular material within said third microdroplet.
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35. A microfluidic system for processing fluids comprising:
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a microfluidic substrate comprising an enrichment zone for preparing an enriched particle sample from a cell-containing fluid, a gas actuator for providing a gas pressure, a first valve for coupling the enrichment zone to the gas actuator, a lysing module for lysing cells to release intracellular material, and a second valve for coupling the enrichment zone to the lysing module, and a control device for controlling the operation of the microfluidic substrate, wherein the control device controls fluid flow by i) closing said first valve to thereby block flow of fluid between the enrichment zone and the gas actuator, and ii) closing said second valve to thereby block fluid flow between the enrichment zone and the lysing module, and wherein the control device controls movement of the enriched particle sample by opening said first and second valves to allow said gas actuator to provide a gas pressure for moving the enriched particle sample from the enrichment zone to said lysing module.
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Specification