Novel modified MSP-1 nucleic acid sequences and methods for increasing mRNA levels and protein expression in cell systems
First Claim
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1. A modified MSP-1 nucleic acid sequence, as shown in SEQ ID NO 1.
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Abstract
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of malarial surface protein MSP-1 which is known to be difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred protein candidates for expression using the recombinant techniques of the invention are MSP-1 proteins expressed from DNA coding sequences comprising reduced overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the native MSP-1 gene.
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8 Claims
- 1. A modified MSP-1 nucleic acid sequence, as shown in SEQ ID NO 1.
- 2. A process for preparing a modified MSP-1 nucleic acid comprising the steps of lowering the overall AT content of the natural gene encoding MSP-1, eliminating all mRNA instability motifs and replacing all rare codons with a preferred codon of the mouse mammary gland.
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