×

OLIGONUCLEOTIDES CONTAINING PYRAZOLO[3,4-D] PYRIMIDINES FOR HYBRIDIZATION AND MISMATCH DISCRIMINATION

  • US 20020146690A1
  • Filed: 11/01/1999
  • Published: 10/10/2002
  • Est. Priority Date: 04/03/1998
  • Status: Active Grant
First Claim
Patent Images

1. A method for distinguishing polynucleotides with related sequences, the method comprising the following steps:

  • (a) providing an oligonucleotide having a defined sequence, wherein one or more purine residues of the oligonucleotide are substituted by a pyrazolo[3,4-d]pyrimidine;

    (b) providing at least two polynucleotides, each of which comprises a target sequence, wherein one of the polynucleotides has a target sequence that is perfectly complementary to the oligonucleotide and at least one other of the polynucleotides has a related target sequence;

    (c) separately incubating each of the polynucleotides with the oligonucleotide under hybridization conditions; and

    (d) determining the degree of hybridization between the oligonucleotide and each of the polynucleotides.

View all claims
  • 5 Assignments
Timeline View
Assignment View
    ×
    ×