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Method of increasing complementarity in a heteroduplex

  • US 20020146732A1
  • Filed: 02/01/2002
  • Published: 10/10/2002
  • Est. Priority Date: 02/02/2001
  • Status: Abandoned Application
First Claim
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1. An in vitro method of making sequence variants from at least one heteroduplex polynucleotide where said heteroduplex has at least two non-complementary nucleotide base pairs, said method comprising:

  • a. preparing at least one heteroduplex polynucleotide;

    b. combining said heteroduplex polynucleotide with an effective amount of CEL I, T4 DNA polymerase, and T4 DNA ligase; and

    c. allowing sufficient time for the percentage of complementarity to increase, wherein one or more variants are made.

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