METHOD FOR PRODUCING HIGHLY UNSATURATED FATTY ACIDS AND LIPID CONTAINING SAME
First Claim
1. A process for producing arachidonic acid comprising the steps of culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source of high concentration, in a medium having a carbon source concentration of at least 4% by weight at the start of culturing, thereby forming arachidonic acid or lipid containing arachidonic acid, and recovering arachidonic acid.
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Abstract
A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
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Citations
30 Claims
- 1. A process for producing arachidonic acid comprising the steps of culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source of high concentration, in a medium having a carbon source concentration of at least 4% by weight at the start of culturing, thereby forming arachidonic acid or lipid containing arachidonic acid, and recovering arachidonic acid.
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2. A process for producing lipid containing arachidonic acid comprising the steps of culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source of high concentration, in a medium having a carbon source concentration of at least 4% by weight at the start of culturing, thereby forming lipid containing arachidonic acid, and recovering lipid containing arachidonic acid.
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8. A process for producing dihomo-γ
- -linolenic acid comprising the steps of culturing a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing and containing a Δ
5 denatures inhibitor, thereby forming dihomo-γ
-linolenic acid or lipid containing dihomo-γ
-linolenic acid, and recovering dihomo-γ
-linolenic acid. - View Dependent Claims (10, 11, 12, 13, 14, 17, 18, 19, 20)
- -linolenic acid comprising the steps of culturing a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing and containing a Δ
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9. A process for producing lipid containing dihomo-γ
- -linolenic acid comprising the steps of culturing a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing and containing a Δ
5 desaturase inhibitor, thereby forming lipid containing dihomo-γ
-linolenic acid, and recovering lipid containing dihomo-γ
-linolenic acid.
- -linolenic acid comprising the steps of culturing a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing and containing a Δ
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15. A process for producing eicosapentaenoic acid comprising the steps of culturing at temperatures of up to 20°
- C. a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing, thereby forming eicosapentaenoic acid or lipid containing eicosapentaenoic acid, and recovering eicosapentaenoic acid.
- View Dependent Claims (21)
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16. A process for producing lipid containing eicosapentaenoic acid comprising the steps of culturing at temperatures of up to 20°
- C., a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration in a medium having a carbon source concentration of at least 4% by weight at the start of culturing, thereby forming lipid containing eicosapentaenoic acid, and recovering lipid containing eicosapentaenoic acid.
- 22. Animal feed comprising microbial cells of a microorganism belonging to the genus Mortierella and having resistance to a carbon source of high concentration.
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30. The genus Mortierella, strain SAM 2197 (FERM BP-6261) having resistance to a carbon source of high concentration.
Specification