Probe carrier, method of manufacturing the same and apparatus to be used for the method
First Claim
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1. A probe carrier having on an elongate substrate a plurality regions fixing different probes each adapted to be bonded specifically to a target substance, said regions fixing probes being arranged longitudinally on said substrate.
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Abstract
A probe carrier of a DNA array or the like is formed by arranging regions fixing different probes in the axial direction of a substrate having an oblong profile which is string-shaped, tape-shaped, string-shaped, tube-shaped or rod-shaped. With this arrangement, the efficiency of manufacturing a probe carrier and handling it in different steps of specimen analysis is improved.
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Citations
40 Claims
- 1. A probe carrier having on an elongate substrate a plurality regions fixing different probes each adapted to be bonded specifically to a target substance, said regions fixing probes being arranged longitudinally on said substrate.
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4. A probe carrier roll formed by winding on a core a probe carrier having on an elongate substrate a plurality regions fixing different probes each adapted to be bonded specifically to a target substance, said regions fixing probes being arranged longitudinally on said substrate.
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5. A cassette for detecting a target substance by means of probes adapted to be bonded specifically to the target substance, said cassette comprising in a cabinet:
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a probe carrier roll containing section containing a probe carrier roll formed by winding on a feed reel a probe carrier having on an elongate substrate a plurality regions fixing different probes each adapted to be bonded specifically to a target substance, said regions fixing probes being arranged longitudinally on said substrate;
a take-up reel for taking up the probe carrier fed out from the feed reel; and
an opening arranged on the moving path of the probe carrier from said probe carrier roll containing section to said take-up reel.
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6. A method for detecting a target substance in a specimen by means of probes adapted to be bonded specifically to the target substance, said method comprising:
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a step of causing a probe carrier having regions fixing different probes to contact with the specimen, the regions being arranged in the longitudinal direction of an elongate substrate; and
a step of detecting presence or absence of a reaction of the target substance with the probe of each of the regions fixing probes caused to contact with the specimen. - View Dependent Claims (7, 8, 9, 10, 11, 13, 14, 15, 16, 18, 19, 20, 21, 22, 23, 25, 26, 27, 29, 30, 31)
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12. A target substance detecting apparatus for detecting presence or absence of a reaction of probes with a target substance in a specimen by detecting a signal generated by the reaction, the probes being adapted to be bonded specifically to the target substance, said apparatus comprising:
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a signal detecting means; and
a moving means for longitudinally moving a sample for observation relative to said detecting means, said sample for observation being obtained by causing the specimen to contact with a probe carrier having regions fixing different probes, said regions being arranged in the longitudinal direction of an elongate substrate.
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17. A method for detecting a target substance in a specimen by means of probes adapted to be bonded specifically to the target substance, said method comprising:
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(1) a step of preparing a probe carrier by arranging regions fixing different probes in the longitudinal direction of an elongate substrate;
(2) a step of obtaining a sample for observation by causing the probe carrier to contact with the specimen; and
(3) a step of detecting presence or absence of a reaction of the target substance with the probe in each of the regions fixing probes in the sample for observation.
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24. A detection apparatus for detecting a reaction of probes adapted to be bonded specifically to a target substance with the target substance in a specimen by detecting presence or absence of a signal generated by the reaction, said apparatus comprising:
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a probe carrier preparing section for preparing a probe carrier carrying probes fixed thereto on an elongate substrate;
a sample preparing section for preparing a sample for observation by causing the probe carrier prepared by said probe carrier preparing section to contact with a specimen; and
a detecting section for detecting a signal generated by the reaction of the probes with a target substance in the sample for observation as prepared by said sample preparing section;
said probe carrier preparing section having;
a means for applying solutions of the probes;
a means for holding the substrate; and
a first moving means for moving said solution applying means longitudinally relative to the substrate;
said detecting section having;
a means for detecting a signal; and
a second moving means for moving said signal detecting means relative to the sample for observation as prepared by causing the probe carrier having regions fixing different probes to contact with the specimen, said regions being arranged in the longitudinal direction of the substrate.
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28. A method for manufacturing a probe carrier having regions for fixing different probes adapted to be bonded specifically to a target substance, said regions being arranged in the longitudinal direction of an elongate substrate, said method comprising:
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a step of moving a means for applying probe solutions relative to said substrate from the front end to the rear end thereof so as to apply different probe solutions as a function of the arrangement of said regions; and
a step of fixing the probes in said regions carrying the respective probe solutions applied thereto to said substrate.
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32. A probe carrier manufacturing apparatus for 4 manufacturing a probe carrier having regions fixing different probes adapted to be bonded specifically to a target substance, said regions being arranged in the longitudinal direction of an elongate substrate, said apparatus comprising:
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a means for applying probe solutions; and
a means for relatively moving said solution applying means from the front end to the rear end of the long substrate. - View Dependent Claims (33, 34, 35, 40)
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36. A probe carrier comprising:
a plurality of regions fixing probes arranged axially in the inside of a tubular member, said regions carrying different probes fixed thereto.
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37. A method for detecting a target substance in a specimen by means of probes adapted to be bonded specifically to a target substance, said method comprising:
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a step of flowing a solution containing a specimen from one of two openings of a tubular member as a probe carrier to the other opening, a plurality of regions fixing probes being arranged axially in the inside of the tubular member, said regions carrying different probes fixed thereto; and
a step of detecting presence or absence of a reaction of the target substance with the probe of each of said regions fixing probes caused to contact with the specimen.
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38. A method of manufacturing a probe carrier having a plurality of regions fixing probes adapted to be bonded specifically to a target substance, said regions being arranged axially in the inside of a tubular member, said regions carrying different probes fixed thereto, said method comprising:
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a step of causing solutions of a plurality of probes necessary for forming and arranging the regions to adhere to a plate-shaped member according to the arrangement of the regions;
a step of solidifying the probes in the regions carrying the probes adhered thereto on the plateshaped member; and
a step of molding the plate-shaped member carrying the solidified probes adhered thereto into a tubular member so as to make the regions fixing probes to be arranged in the axial direction on the inner wall of the tubular member.
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39. A method for detecting a target substance in a specimen by means of probes adapted to be bonded specifically to a target substance, said method comprising:
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a step of flowing a solution containing the specimen into the inside of a tubular member having regions fixing probes arranged in the axial direction in the inside thereof and causing the solution to contact with each of the regions fixing probes to obtain a sample for observation; and
a step of detecting presence or absence of a reaction of the target substance with the probe of each of the regions fixing probes in the sample for observation.
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Specification