Method of measurement in chromatography

• US 20020150930A1
• Filed: 05/01/2002
• Published: 10/17/2002
• Est. Priority Date: 05/08/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Tanaka, Hirotaka, Takahashi, Mie, Nadaoka, Masataka
• Granted Patent: US 7,192,784 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: G01N 33/689 related to pregnancy or the gonads
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A substantial amount of a marker reagent associated with measurement is measured in an marker reagent measurement area 6, and the substantial amount of the marker reagent is reflected by a result obtained in an reaction result measurement area 7 where a reaction with a measurement target in an inspection target solution is measured.

Thereby, an influence of external environmental factors, factors in a property of the inspection target solution, an erroneous operation in a measurement operation or the like is eliminated, whereby a chromatography measuring method employing a biosensor with a higher accuracy and a higher reliability can be provided.

Claims

1. A chromatography measuring method for measuring components to be measured included in an inspection target solution utilizing a chromatography, with employing a biosensor comprising a development layer where the inspection target solution is developed, a reagent immobilization part formed by immobilizing a reagent in a part of the development layer, and a marker reagent hold part formed by holding a marker reagent in another part of the development layer so as to be eluted by the development of the inspection target solution, wherein

a bonding amount of the marker reagent in the reagent immobilization part is measured, thereby determining the quality or quantity of the components to be measured in the inspection target solution, and

an amount of eluted marker reagent components or residual marker reagent components which have not been eluted is measured.

2. The chromatography measuring method as defined in claim 1, wherein

the bonding amount of the marker reagent in the reagent immobilization part is corrected employing the amount of the eluted marker reagent components or residual marker reagent components which have not been eluted.

3. The chromatography measuring method as defined in claim 1 or 2, wherein

the measurement of the amount of the eluted marker reagent components or residual marker reagent components which have not been eluted employs an optical detector.

4. The chromatography measuring method as defined in any of claims 1 to 3, wherein

the measurement of the amount of the eluted marker reagent components is performed in a part other than the reagent immobilization part.

5. The chromatography measuring method as defined in any of claims 1 to 4, wherein

the measurement of the amount of the eluted marker reagent components is performed before the bonding amount of the marker reagent is measured in the reagent immobilization part.