Novel differentiation inducing process of embryonic stem cell to ectodermal cell and its use
First Claim
1. A method for inducing differentiation of an embryonic stem cell into an ectodermal cell, which comprises culturing the embryonic stem cell under non-aggregation conditions.
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Abstract
A method for inducing differentiation of an embryonic stem cell into an ectodermal cell and an ectoderm-derived cell, which comprises culturing the embryonic stem cell under non-aggregation conditions; a medium and a medium supernatant used in the method; an agent for inducing differentiation used in the method; a stroma cell or a stroma cell-derived factor having activity of inducing differentiation in the method; an antibody which specifically recognizes the stroma cell; an antigen which recognizes the antibody; a cell induced by the method; a method for evaluating or screening a substance relating to the regulation in a differentiation step from an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell by carrying out the method; and a medicament comprising the stroma cell, the stroma cell-derived cell, the antibody, the antigen or the cell.
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Citations
71 Claims
- 1. A method for inducing differentiation of an embryonic stem cell into an ectodermal cell, which comprises culturing the embryonic stem cell under non-aggregation conditions.
- 3. A method for inducing differentiation of an embryonic stem cell into an ectoderm-derived cell, which comprises culturing the embryonic stem cell under non-aggregation conditions.
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29. A stroma cell-derived factor which induces differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell.
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32. An agent for inducing differentiation of an ectodermal cell into an epidermal system cell, which comprises, as an active ingredient, bone morphogenetic protein 4.
- 33. An agent for inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell, which comprises, as an active ingredient, a stroma cell which has activity of inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell, or a factor derived from the cell.
- 37. A medium which comprises a culture supernatant obtained by culturing a stroma cell which has activity of inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell in a medium comprising a mucopolysaccharide.
- 41. A method for obtaining an antibody which specifically recognizes a stroma cell which has activity of inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell, which comprises using a stroma cell as an antigen.
- 44. A monoclonal antibody produced by a hybridoma FERM BP-7573.
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48. A method for obtaining a stroma cell-derived factor which has activity of inducing differentiation of an embryonic stem cell into an ectoderm-derived cell, which comprises using, as an index, the activity of inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell.
- 60. A medicament comprising a stroma cell having activity of inducing differentiation of an embryonic stem cell into an ectodermal cell or an ectoderm-derived cell, or a factor derived from the cell.
Specification