×

Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction

  • US 20020155439A1
  • Filed: 12/04/2000
  • Published: 10/24/2002
  • Est. Priority Date: 12/04/2000
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of producing a library of mutant nucleic acid molecules comprising:

  • (a) obtaining a template nucleic acid;

    (b) preparing a first oligonucleotide corresponding to a first desired mutation within said template nucleic acid;

    (c) preparing a second oligonucleotide corresponding to a second desired mutation within said template nucleic acid;

    (d) mixing the oligonucleotides prepared in said steps (b) and (c) so as to hybridize said oligonucleotides to said template nucleic acid;

    (e) subjecting the mixture of step (d) to the linear cyclic amplification reaction to produce a library of mutant template nucleic acids.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×