Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction

• US 20020155439A1
• Filed: 12/04/2000
• Published: 10/24/2002
• Est. Priority Date: 12/04/2000
• Status: Abandoned Application

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Rodriguez, Ana, Schellenberger, Volker, Wang, Huaming
• Application Number:
  US09/729,520
• Publication Number:
  US 20020155439A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/102 Mutagenizing nucleic acids

Abstract

The present invention provides for a method of producing mutant nucleic acid molecules comprising preparing a first and second oligonucleotide corresponding to two different mutations in a template nucleic acid, mixing the oligonucleotides with a template to which they correspond so as to hybridize and subjecting the mixture to the linear cyclic amplification reaction. The present invention is particularly well suited for the development of libraries of mutant nucleic acids.

Claims

1. A method of producing a library of mutant nucleic acid molecules comprising:

(a) obtaining a template nucleic acid;

(b) preparing a first oligonucleotide corresponding to a first desired mutation within said template nucleic acid;

(c) preparing a second oligonucleotide corresponding to a second desired mutation within said template nucleic acid;

(d) mixing the oligonucleotides prepared in said steps (b) and (c) so as to hybridize said oligonucleotides to said template nucleic acid;

(e) subjecting the mixture of step (d) to the linear cyclic amplification reaction to produce a library of mutant template nucleic acids.

2. The method according to claim 1, wherein said oligonucleotides in said steps (b) and (c) are discontiguous.

3. The method according to claim 1, wherein said step first and second oligonucleotides are present in less than saturation concentration.

4. The method according to claim 1, wherein the mixture of said step (d) further comprises non-mutagenic oligonucleotides corresponding to either or both of said first and second oligonucleotides.

5. The method according to claim 1, wherein said template nucleic acid corresponds to a desired protein product.

6. The method according to claim 4, wherein said protein product comprises an enzyme, hormone, vaccine, peptide therapeutic or antibody.

7. The method according to claim 4, further comprising the steps of:

(f) transforming said mutant template nucleic acids from said library into a competent host cell;

(g) expressing protein corresponding to said mutant nucleic acids in said host cell;

(h) screening said expressed proteins for desired characteristics.