Separation unit, a method for separation, and a device for mounting a separation unit in a separation apparatus

• US 20020158016A1
• Filed: 04/26/2002
• Published: 10/31/2002
• Est. Priority Date: 04/26/2001
• Status: Abandoned Application

• Current Assignee: ESyTech AB
• Original Assignee: ESyTech AB
• Inventors: Norberg, Jan, Thordarson, Eddie, Soderlund, Patrik, Yazarlo, Shadi, Hakansson, Fredik, Larsson, Lars-Ake, Berhin, Johan
• Application Number:
  US10/132,737
• Publication Number:
  US 20020158016A1
• Time in Patent Office: Days
• US Class Current: 210/634
• CPC Class Codes: G01N 1/4005 by transferring a selected component through a membrane
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis
• CPC Class Codes: G01N 2030/204 Linearly moving valves, e.g. sliding valves
• CPC Class Codes: G01N 30/20 using a sampling valve
• CPC Class Codes: G01N 30/6047 with supporting means; Holders
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A modular separation system wherein a separation unit is separate from a mounting device that accommodates the separation unit in a separation apparatus. The mounting device is an integral part of the separation apparatus. A method for fluid-fluid extraction of an analyte from a sample fluid to a receiving fluid in a separation apparatus.

Claims

1. A separation unit to be put into position by insertion in a mounting device for use in separation of an analyte from a sample fluid to a receiving fluid, the unit comprising:

a two part body portion with at least one sample fluid cavity for accommodating sample fluid, and at least one receiving fluid cavity for accommodating receiving fluid, whereby each sample fluid cavity is connected to its corresponding receiving fluid cavity,

each sample fluid cavity comprises a sample fluid inlet for leading sample fluid into the sample fluid cavity, and a sample fluid outlet for leading sample fluid out of the sample fluid cavity,

each receiving fluid cavity comprises a receiving fluid inlet for leading receiving fluid into the receiving fluid cavity, and a receiving fluid outlet for leading receiving fluid out of the receiving fluid cavity.

2. A separation unit according to claim 1 which includes at least one separation medium.

3. A separation unit according to claim 1, wherein the body portion defines upper and lower surfaces and at least one rim or edge portion, and wherein the ratio between a maximal height of the rim or edge portion and a maximal diagonal dimension of the upper and lower surface is at most 1 to 4.

4. A separation unit according to claim 2, wherein the separation medium is a membrane support comprising a sheet or strip arranged between a first and a second wall member.

5. A separation unit according to any of claim 1, wherein the first and second wall members are identical.

6. A separation unit according to any of claim 1, which contains more than one sample fluid cavity and more than one receiving fluid cavity.

7. A separation unit according to any of claim 1, wherein a surface of at least one of the wall members has a projecting portion, and wherein at least one of the grooves or cut-outs is formed in the projecting portion, whereby the projecting portion contributes to fix the membrane support in an operating position between the wall members.

11. A separation system for fluid-fluid extraction comprising a separation unit according to claim 1.

13. A method for fluid-fluid extraction of an analyte from a sample fluid to a receiving fluid in a separation apparatus according to claim 12, comprising the steps of:

leading a receiving fluid through the receiving fluid inlet into the receiving fluid cavity,

continuously leading a sample fluid through the sample fluid inlet into the sample fluid cavity and out of the sample fluid outlet,

leading the receiving fluid out of the receiving fluid cavity through the receiving fluid outlet.

14. A method according to claim 13, wherein the sample fluid and the receiving fluid contact the separation medium simultaneously.

15. A method according to claim 13, wherein the sample fluid and the receiving fluid contact the separation medium sequentially.

16. A method according to any of claim 13, wherein, subsequent to leading an amount of sample fluid out of the sample fluid cavity, an amount of sample fluid is led back into the sample fluid cavity through the sample fluid inlet or back through the sample fluid outlet.

17. A method according to any of claim 13, wherein the receiving fluid outlet is connected directly to an apparatus for analysing an analyte in the receiving fluid.

18. A method according to any of claim 13, wherein the steps of the method are repeated with one separation unit.

19. A method according to any of claim 13, wherein the separation unit is replaced with a new separation unit after one separation.

20. The use of a separation system according to claim 11 for molecular diffusion.

21. The use of claim 20, wherein the diffusion comprises dialysis.

22. The use of a separation system according to claim 11 for molecular extraction.

8. A device for mounting a separation unit in a separation apparatus, the device comprising:

a receiving fluid injector connectable to the receiving fluid inlet,

a receiving fluid extractor connectable to the receiving fluid outlet,

a sample fluid injector connectable to the receiving fluid inlet,

a sample fluid extractor connectable to the receiving fluid outlet.

9. A device according to claim 8, wherein the device comprises a control system for controlling positioning of at least the receiving fluid extractor in relation to the receiving fluid outlet.

10. A device according to claim 8, wherein the device comprises a control system for controlling positioning of at least the sample fluid injector in relation to the sample fluid inlet.

12. A separation apparatus for fluid-fluid extraction comprising means for introducing sample fluid to a mounting device according to claim 8.