Integrated monolithic microfabricated electrospray and liquid chromatography system and method
First Claim
1. An integrated miniaturized system for chemical analysis of fluids, comprising:
- an electrospray substrate having an injection side and an ejection surface, the substrate defining an entrance orifice on the injection side, a nozzle on the ejection surface, a channel extending between the entrance orifice and the nozzle, and a region surrounding the nozzle recessed from the ejection surface; and
electrode means for providing electrical contact to the fluids.
2 Assignments
0 Petitions
Accused Products
Abstract
An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.
-
Citations
22 Claims
-
1. An integrated miniaturized system for chemical analysis of fluids, comprising:
-
an electrospray substrate having an injection side and an ejection surface, the substrate defining an entrance orifice on the injection side, a nozzle on the ejection surface, a channel extending between the entrance orifice and the nozzle, and a region surrounding the nozzle recessed from the ejection surface; and
electrode means for providing electrical contact to the fluids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. An electrospray device comprising:
-
an array of nozzles integrated in a substrate for ejecting a plurality of analytes at a mass spectrometry device interface; and
a plurality of electrodes for the application of electric potentials for generating and controlling an electric field at each nozzle to direct the ejection of the analytes from the nozzles within an acceptance region of the mass spectrometry device.
-
-
11. A method for generating an electrospray of a fluid, comprising:
-
providing a channel extending between an entrance orifice defined on an injection surface of a substrate and a nozzle defined on an ejection surface of the substrate;
introducing a fluid into the channel through the entrance orifice;
providing a first electrode in electrical contact with the fluid;
applying a first potential voltage to the fluid;
positioning the nozzle adjacent to an extracting electrode; and
ejecting the fluid from the channel through the nozzle by applying or holding the extracting electrode at a second potential voltage different from the first potential voltage. - View Dependent Claims (12, 14, 15, 16, 19, 20)
-
-
13. A liquid chromatography system, comprising:
-
a first substrate having a first surface and a second surface, the first substrate defining an entrance opening on the first surface, a fluid reservoir recessed from the second surface, a first channel extending between the entrance opening and the reservoir, a second channel recessed from the second surface, and a plurality of posts extending from the second channel; and
a cover substrate bonded to the first substrate to enclose the reservoir and the second channel adjacent the cover substrate, wherein the first and/or the cover substrate defines an exit and wherein the second channel extends between the exit and the reservoir.
-
-
17. A chemical separation device comprising:
-
a substrate defining a channel, a plurality of posts fabricated from said substrate and extending from said channel, and a stationary phase bound to the posts, said posts providing interaction with an analyte introduced into said channel for producing separation.
-
-
18. An integrated chemical analysis system, comprising:
-
a first substrate having a major surface;
a second substrate bonded to or otherwise attached to said first substrate;
a liquid chromatography system integrated in said second substrate, and configured to receive a fluid for analysis and to process and output the fluid; and
an electrospray device integrated on said first substrate, the electrospray device having an injection surface configured to receive the processed fluid from the liquid chromatography system, the major surface being configured to dispense the fluid by electrospraying the fluid.
-
-
21. A system comprising:
-
a microfabricated device defining a liquid chromatography device comprising an entrance for receiving an analyte, the device further defining an electrospray device including a nozzle, the electrospray device being configured to receive the analyte from the liquid chromatography device and to generate an electrospray; and
a mass spectrometer comprising a sampling orifice, said microfabricated device being positioned to eject the electrospray from the nozzle into the sampling orifice.
-
-
22. A method of mass spectrometric analysis utilizing an integrated chemical analysis device comprising:
-
a first microfabricated structure defining a liquid chromatography device comprising an entrance for receiving an analyte and an exit; and
a second microfabricated structure defining an electrospray device including an entrance for receiving the analyte from the liquid chromatography device and a nozzle in fluid communication with the entrance, the electrospray device to generate an electrospray and wherein the electrospray nozzle is adapted to eject the electrospray from the nozzle into a sampling orifice of a mass spectrometer.
-
Specification