Process for purifying antibody

• US 20020164328A1
• Filed: 10/04/2001
• Published: 11/07/2002
• Est. Priority Date: 10/06/2000
• Status: Active Grant

• Current Assignee: Kyowa Kirin Co., Ltd. (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Inventors: Shinkawa, Toyohide, Uchida, Kazuhisa, Yamasaki, Motoo, Hosaka, Emi, Shitara, Kenya
• Granted Patent: US 7,064,191 B2
• Time in Patent Office: Days
• US Class Current: 424/130.1
• CPC Class Codes: C07K 16/065 Purification, fragmentation
• CPC Class Codes: C07K 16/2866 against receptors for cytokines, lymphokines, interferons
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/732 Antibody-dependent cellular cytotoxicity [ADCC]
• CPC Class Codes: Y10S 436/824 Immunological separation techniques
• CPC Class Codes: Y10S 436/827 Lectins

Abstract

The present invention relates to a process for purifying an antibody having a desired property, which comprises using a substance having an affinity to a carbohydrate binding to the antibody; a medicament comprising, as an active ingredient, the antibody purified by the process; and a method for diagnosing or preventing various diseases, which comprises using a substance having an affinity to a carbohydrate binding to an antibody.

Claims

1. A process for purifying an antibody having a desired property, which comprises using a substance having an affinity to a carbohydrate binding to the antibody.

2. The process according to claim 1, wherein the carbohydrate is an N-glycan.

3. The process according to claim 2, wherein the N-glycan is a carbohydrate to which bisecting N-acetylglucosamine, fucose or galactose is bound.

4. The process according to claim 1, wherein the substance having an affinity to a carbohydrate is a lectin.

5. The process according to claim 4, wherein the lectin is at least one lectin selected from the group consisting of a concanavarin A, a wheat germ lectin, a Lens culinaris lectin and a Lens culinaris lectin E₄.

6. The process according to claim 1, wherein the substance having an affinity to the carbohydrate is bound to a carrier.

7. The process according to claim 6, wherein the carrier is a synthetic resin polymer.

15. A process for purifying an antibody having a desired property, which comprises combining the process according to any one of claims 1 to 14.

16. The process according to any one of claims 1 to 15, wherein the antibody is human IgG.

18. A medicament comprising, as an active ingredient, the antibody purified by the process according to any one of claims 1 to 17.

8. A process for purifying an antibody having a carbohydrate structure to which bisecting N-acetylglucosamine is bound, which comprises using a column to which a wheat germ lectin or a Lens culinaris lectin E₄ is immobilized.

9. A process for purifying an antibody having a high antibody-dependent cell-mediated cytotoxic activity, which comprises using a column to which a wheat germ lectin or a Lens culinaris lectin E₄ is immobilized.

10. A process for purifying an antibody having a carbohydrate structure to which fucose is bound, which comprises using a column to which a Lens culinaris lectin is immobilized.

11. A process for purifying an antibody having a high antibody-dependent cell-mediated cytotoxic activity, which comprises using a column to which a Lens culinaris lectin is immobilized.

14. The process according to claim 13, wherein a phenyl group is bound to the carrier for hydrophobic chromatography.

17. The process according to claim 16, wherein the subclass of the human IgG is IgG1.

19. The medicament according to claim 18, wherein the antibody is human IgG.

20. The medicament according to claim 19, wherein the subclass of the human IgG is IgG1.

12. A process for purifying an antibody having a carbohydrate structure to which galactose is bound, which comprises using a carrier for hydrophobic chromatography.

13. A process for purifying an antibody having a high complement-dependent cytotoxic activity or antibody-dependent cell-mediated cytotoxic activity, which comprises using a carrier for hydrophobic chromatography.

21. A method for diagnosing various diseases, which comprises using a substance having an affinity to a carbohydrate binding to an antibody.