Analytical methods and compositions
First Claim
1. A composition for the detection of one or more target entities in a mixture, said composition comprising non-magnetic microparticles or beads operably linked with a first binding partner capable of binding to a complementary second binding partner, said second binding partner being operably linked to magnetic particles smaller than the average size of said non-magnetic microparticles, thereby to form a layer of magnetic particles on said non-magnetic microparticles, wherein said magnetic particles bear variable amounts of free binding sites distal to the surface of said non-magnetic microparticles and wherein the bound magnetic particles are capable of binding both a labeled first binding partner and said first binding partner either in its free form or operably linked to one or more entities including entities for capturing said target entities in the mixture.
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Accused Products
Abstract
Compositions and analytical methods for detection of a single target entity or for simultaneous detection of multiple target entities by means of non-magnetic microparticles operably linked with a first binding partner capable of binding to a complementary second binding partner which, in turn, is operably linked to smaller magnetic particles to form a layer of magnetic particles on the non-magnetic microparticles.
102 Citations
17 Claims
- 1. A composition for the detection of one or more target entities in a mixture, said composition comprising non-magnetic microparticles or beads operably linked with a first binding partner capable of binding to a complementary second binding partner, said second binding partner being operably linked to magnetic particles smaller than the average size of said non-magnetic microparticles, thereby to form a layer of magnetic particles on said non-magnetic microparticles, wherein said magnetic particles bear variable amounts of free binding sites distal to the surface of said non-magnetic microparticles and wherein the bound magnetic particles are capable of binding both a labeled first binding partner and said first binding partner either in its free form or operably linked to one or more entities including entities for capturing said target entities in the mixture.
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15. A composition useful in the calibration of a diagnostic instrument system which utilizes fluorescent detection, said composition comprising magnetic particles bound to particles having a detectable fluorescent label, said magnetic particles having an average size less than that of the particles having a detectable fluorescent label.
Specification