Primitive neural stem cells and method for differentiation of stem cells to neural cells
First Claim
20. A method for producing secondary neural stem cell colonies comprising:
- (a) culturing ES cells in low cell density completely defined serum-free media for a time and under conditions sufficient to differentiate the said ES cells;
(b) dissociating and subcloning primary neural cell colonies generated from the said ES cells; and
(c) administering a growth factor to the dissociated neural cells.
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Abstract
Described are a novel cell type in the neural lineage, and method of producing the same based on the degree of neural commitment and growth factor responsiveness in vitro and the potential to give rise to neural and non-neural progeny in vivo. The novel veil type of neural lineage and cells derived therefrom have a number of applications including applications regarding tissue engineering, transplantation and gene therapy and drug discovery. Also described are suggested uses of the method and cell type including isolating genes that positively and negatively regulate the transmission from an ES cell to a neural cell and generally for studying ES cell models of mammalian neural development.
66 Citations
45 Claims
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20. A method for producing secondary neural stem cell colonies comprising:
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(a) culturing ES cells in low cell density completely defined serum-free media for a time and under conditions sufficient to differentiate the said ES cells;
(b) dissociating and subcloning primary neural cell colonies generated from the said ES cells; and
(c) administering a growth factor to the dissociated neural cells. - View Dependent Claims (21, 22, 23, 24, 26, 27, 37, 43, 44, 45)
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25. One or more cell(s) expressing one or more neural precursor cell marker(s) and/or one or more neural-specific mRNA molecule(s), and having multilineage potential.
- 30. A primitive neural stem cell produced comprising at least one neural cell marker and is pluripotent.
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33. A method for screening for modulators of cellular differentiation comprising
(a) culturing pluripotent cells in serum-free media under low density conditions in the presence of the potential modulator; -
(b) allowing for differentiation of the cells;
(c) detecting any differentiation of the cells and cell types generated, if any. - View Dependent Claims (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 28, 29, 34, 36, 38, 39, 40, 41, 42)
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35. A method for screening for differentiation factors of cellular development comprising:
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(a) culturing the cells in serum free media at low cell density in the presence of the differentiation factor;
(b) allowing cells to differentiate;
(c) detecting differentiation of the cells, if any.
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38-1. A modulator or differentiation factor detected by the methods of claims 33-37.
Specification