Cell separation matrix
First Claim
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1. A matrix for detecting the presence of cancer cells in a subject, said matrix comprising a solid core material bearing a coating having binding affinity for said solid core material and binding affinity for blood-borne adhesion components that bind cancer cells, and one or more blood-borne adhesion components.
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Abstract
A novel modified matrix system, mimicking a metastatic environment, that can be used to capture and detect viable cancer and normal cells from tissue fluid samples derived from cancer subjects and which provides effective cell separation for diagnostic and therapeutic applications in treating patients with metastatic diseases.
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Citations
34 Claims
- 1. A matrix for detecting the presence of cancer cells in a subject, said matrix comprising a solid core material bearing a coating having binding affinity for said solid core material and binding affinity for blood-borne adhesion components that bind cancer cells, and one or more blood-borne adhesion components.
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6. A metastatic cancer cell separation system comprising:
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a sealable container having an outer surface and an inner surface;
an adhesion binding material coated on said inner surface of said sealable container, said adhesion binding material having the ability to bind said inner surface of said sealable container and the ability to bind one or more natural or synthetic molecules that have a binding affinity for metastatic cancer cells; and
one or more natural or synthetic molecules having a binding affinity for metastatic cancer cells. - View Dependent Claims (7, 8, 9, 10)
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12. A metastatic cancer cell separation system comprising:
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a sealable container having an outer surface and an inner surface, said inner surface defining a void;
a plurality of beads coated with an adhesion binding material bound to one or more natural or synthetic molecules that have a binding affinity for metastatic cancer cells, said beads residing within said void;
a separation member positioned in said void in such a manner as to divide said void into two or more compartments said filter having pores of a size to permit filtration of said beads.
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20. A metastatic cancer cell separation system comprising:
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a sealable container having an outer surface and an inner surface, said inner surface defining a void;
a plurality of magnetically-attractable microbeads or nanoparticles coated with an adhesion binding material bound to one or more natural or synthetic molecules that have a binding affinity for metastatic cancer cells, said microbeads or nanoparticles residing within said void; and
a magnet on the outer surface of said sealable container, or within said void, of sufficient strength to attract said plurality of magnetic-attractable microbeads or nanoparticles to one location. - View Dependent Claims (21, 22, 23, 24, 25, 26, 28, 29, 30, 31, 32, 33, 34)
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27. A metastatic cancer cell separation system comprising:
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an enclosed container defining a void, said enclosed container having an inlet and an outlet;
a first separation member positioned proximal to said inlet within said void and dividing said void into compartments, said first separation member permitting the flow of at least a component of whole blood therethrough;
a second separation member positioned proximal to said outlet within said void and dividing said void into compartments, said second separation member permitting the flow of at least a component of whole blood therethrough and being positioned antepodal to said first separation member in said void;
a plurality of beads coated with an adhesion binding material bound to one or more natural or synthetic molecules that have a binding affinity for metastatic cancer cells, said beads residing between said first separation member and second separation member and being retained thereby within said void.
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Specification