Indian green mussel (Perna viridis) as a source of anti-HIV activity

• US 20020168416A1
• Filed: 03/29/2002
• Published: 11/14/2002
• Est. Priority Date: 03/30/2001
• Status: Active Grant

• Current Assignee: Department of Biotechnology and Council of Scientific and Industrial Research
• Original Assignee: Department of Biotechnology and Council of Scientific and Industrial Research
• Inventors: Mitra, Debaslis, Chatterji, Anil
• Granted Patent: US 6,770,302 B2
• Time in Patent Office: Days
• US Class Current: 424/547
• CPC Class Codes: A61K 35/618 Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs

Abstract

The present invention relates to the use of Indian Green Mussel (Perna viridis) as a source of anti-HIV activity. More particularly, the present invention relates to the use of an extract of Indian Green Mussel (Perna viridis) as a source of anti-HIV activity in vitro.

Claims

1. Use of extract of Indian green mussel (Perna viridis) as a source of anti-HIV activity in vitro.

2. Use as claimed in claim 1 wherein the extract comprises the mussel hydrolysate.

3. Use as claimed in claim 1 wherein the mussel hydrolysate is used in 1:

200 dilution.

4. Use as claimed in claim 1 wherein the anti-HIV activity is in vitro, which comprises preparing an extract from the Indian green mussel (Perna viridis), establishing non-toxicity at the dilution (1:

200) on human T-lymphocyte cell line CEM using MTT assay, assessment production of virus in supernatants of the treated and untreated infected cells by p24 antigen ELISA using HIV-1 NL4.3 isolate in T-cell lines CEM and Jurket and using HIV-1 IIIB isolate in monocytic cell line U937.

5. Use as claimed in claim 1 wherein the extract is found to be non-toxic at the dilution (1:

200) on human T-lymphocytic cell line CEM using MTT assay.

6. Use as claimed in claim 1 wherein the supernatants of the treated and untreated infected cells is subjected to p24 antigen ELISA to assess the production of virus using IIIV-1 NL4.3 isolate in T-cell lines CEM and Jurket and using HIV-1 IIIB isolate in monocytic cell line U937.