×

Sequencing duplex DNA by mass spectroscopy

  • US 20020168642A1
  • Filed: 03/26/2001
  • Published: 11/14/2002
  • Est. Priority Date: 06/06/1994
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of sequencing a nucleic acid of interest comprising:

  • providing four populations of pluralities of duplex nucleic acids, each nucleic acid having a common end and a terminal base at the other end, and a length corresponding to the position of the terminal base in the nucleic acid of interest, the duplex nucleic acids having an ionization target, and a detection label associated with the termination base, ionizing the ionizing targets of the populations of duplex nucleic acid with an ionizing agent, fractionating the populations of duplex nucleic acid using mass spectroscopy, for each duplex nucleic acid, resolving a single ionization state, identifying the terminal base by means of the detection label, and determining the sequence length based on mass.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×