USE OF FLP RECOMBINASE IN MICE
First Claim
1. A transgenic mouse comprising a Flp transgene integrated in a genome of the transgenic mouse, wherein the Flp transgene is expressed in a cell of the transgenic mouse at a level of recombinase activity sufficient to catalyze recombination between Flp-recognition sequences of the cell.
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Accused Products
Abstract
A method is disclosed for producing site-specific recombination of DNA in a transgenic non-human mammal at chromosomal regions containing Flp-recognition sites (e.g., a DNA sequence containing an FRT site). The invention in particular discloses the use of site-specific recombinases such as Flp recombinase to accomplish in vivo recombination at engineered chromosomal FRT sites, thereby forming the basis of a genetic system to mark cell populations and lineages, as well as to activate, delete, mutate, or rearrange genes in vivo. DNA constructs are provided for the creation of Flp and FRT transfected eukaryotic cells or transgenic non-human mammals.
11 Citations
49 Claims
- 1. A transgenic mouse comprising a Flp transgene integrated in a genome of the transgenic mouse, wherein the Flp transgene is expressed in a cell of the transgenic mouse at a level of recombinase activity sufficient to catalyze recombination between Flp-recognition sequences of the cell.
- 22. A transgenic mouse comprising a Flp transgene, wherein the Flp transgene is expressed in a cell of the transgenic mouse at a level of recombinase activity sufficient to catalyze recombination between Flp-recognition sequences of the cell.
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23. A transgenic mouse comprising a genome which contains a Flp transgene and a Flp-recognition sequence.
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24. A method of in vivo genetic engineering comprising:
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(a) providing a transgenic mouse comprising a genome which contains a Flp transgene and at least two Flp-recognition sequences, (b) expressing the Flp transgene at a level of recombinase activity sufficient to catalyze site-specific recombination in a cell, and (c) catalyzing recombination between the two Flp-recognition sequences of the cell.
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Specification