Device and method of use for detection and characterization of pathogens and biological materials
First Claim
1. A method of detecting a target pathogen, comprising the steps of:
- providing a substrate with a surface;
depositing a patterned gold layer on the surface;
depositing a deposition material on the surface, the deposition material capable of interacting with the target pathogen; and
exposing the deposition material to a target sample which can contain the target pathogen and detecting resultant molecular interaction events between the deposition material and the target pathogen by imaging the surface with an atomic force microscope.
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Abstract
The present invention includes a method and apparatus for the detection of a target material. The method and apparatus includes providing a substrate with a surface and forming a domains of deposited materials thereon. The deposited material can be placed on the surface and bound directly and non-specifically to the surface, or it may be specifically or non-specifically bound to the surface. The deposited material has an affinity for a specific target material. The domains thus created are termed affinity domains or deposition domains. Multiple affinity domains of deposited materials can be deposited on a single surface, creating a plurality of specific binding affinity domains for a plurality of target materials. Target materials may include, for example, pathogens or pathogenic markers such as viruses, bacteria, bacterial spores, parasites, prions, fungi, mold or pollen spores. The device thus created is incubated with a test solution, gas or other supporting environment suspected of containing one or more of the target materials. Specific binding interactions between the target materials and a particular affinity domain occurs and is detected by various methods.
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Citations
40 Claims
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1. A method of detecting a target pathogen, comprising the steps of:
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providing a substrate with a surface;
depositing a patterned gold layer on the surface;
depositing a deposition material on the surface, the deposition material capable of interacting with the target pathogen; and
exposing the deposition material to a target sample which can contain the target pathogen and detecting resultant molecular interaction events between the deposition material and the target pathogen by imaging the surface with an atomic force microscope. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 27, 28, 29, 31, 32, 33, 34, 35, 36, 38, 39, 40)
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26. An apparatus for the detection of a vaccinia virus, comprising
a gold layer deposited on a substrate; -
an C(x) alkane linker covalently attached to the gold layer;
a protein A/G tethered to the surface through he alkane linker; and
anti-vaccinia antibody deposited on the surface in a domain such that a portion of the deposited antibody retains activity.
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30. The apparatus of claims 30 wherein the control is anti-adenovirus antibody.
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37. A method of detecting a target pathogen, comprising the steps of:
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providing a substrate with a surface;
depositing a deposition material on the surface, the deposition material capable of interacting with the target pathogen;
exposing the deposition material to a target sample which can contain the target pathogen and detecting resultant molecular interaction events between the deposition material and a target pathogen.
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Specification