Method and system for nucleic acid sequencing
First Claim
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1. A method of nucleic acid sequencing comprising the steps:
- (a) amplifying a nucleic acid sample to produce an amplified DNA product;
(b) extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products;
(c) detecting a total amount of label present in the collection to produce a measurement; and
(d) combining a plurality of measurements to determine DNA sequence information about the sample.
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Abstract
A method of nucleic acid sequencing comprising the steps (a) amplifying a nucleic acid sample to produce an amplified DNA product; (b) extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products; (c) detecting a total amount of label present in the collection to produce a measurement; and (d) combining a plurality of measurements to determine DNA sequence information about the sample. A system for nucleic acid sequencing which uses terminating nucleotide analogs to quantitatively determine fragment length and sequence information.
32 Citations
16 Claims
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1. A method of nucleic acid sequencing comprising the steps:
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(a) amplifying a nucleic acid sample to produce an amplified DNA product;
(b) extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products;
(c) detecting a total amount of label present in the collection to produce a measurement; and
(d) combining a plurality of measurements to determine DNA sequence information about the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A system for nucleic acid sequencing comprising:
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(a) a means for amplifying a nucleic acid sample to produce an amplified nucleic acid product;
(b) a means for extending a sequencing primer bound to the DNA product in the presence of terminating nucleotide analogs to produce a collection of labeled nucleic acid products, said extending means in connection with the amplified product;
(c) a means for detecting a total amount of label present in the collection to produce a measurement, said detecting means in connection with the collection; and
(d) a means for combining a plurality of measurements to determine DNA sequence information about the sample, said combining means in connection with the measurement. - View Dependent Claims (12, 14, 15, 16)
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13. A method for obtaining information about a signal comprising the steps:
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(a) inducing a decay function;
(b) imposing the decay function on a signal;
(c) forming a numerical quantity that characterizes the signal'"'"'s behavior in the presence of the decay function;
(d) combining a plurity of such numerical quantities to obtain information about the signal.
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Specification