Identification of non-covalent complexes by mass spectrometry
First Claim
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1. A method for identifying compounds that bind to a target of interest, comprising:
- (a) assembling a first set of target binding ligands that compete for non-covalent binding to a first binding site on the target;
(b) assembling a second set of target binding ligands that compete for non-covalent binding to a second binding site on the target;
(c) chemically linking at least one member of the first set and at least one member of the second set to provide a first set of linked ligands; and
(d) screening the set of linked ligands to identify members thereof that bind to the target.
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Abstract
Methods for identifying novel drug leads or binding compounds that have an affinity for a target molecule involving screening known drug fragment molecules and derivatives thereof, preferably using mass spectrometry are disclosed.
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20 Claims
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1. A method for identifying compounds that bind to a target of interest, comprising:
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(a) assembling a first set of target binding ligands that compete for non-covalent binding to a first binding site on the target;
(b) assembling a second set of target binding ligands that compete for non-covalent binding to a second binding site on the target;
(c) chemically linking at least one member of the first set and at least one member of the second set to provide a first set of linked ligands; and
(d) screening the set of linked ligands to identify members thereof that bind to the target. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 20)
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- 15. A method of preparing a drug lead compound that binds to a target, comprising covalently linking at least one member of a first set of target binding ligands that compete for non-covalent binding to a first binding site on the target and at least one member a second set of target binding ligands that compete for non-covalent binding to a second binding site on the target set to provide a first set of linked ligands.
Specification