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Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems

  • US 20020172980A1
  • Filed: 02/28/2002
  • Published: 11/21/2002
  • Est. Priority Date: 11/27/2000
  • Status: Abandoned Application
First Claim
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1. A method for identifying whether a target agent is present in a biological sample, the method comprising the steps of:

  • preparing a plurality of capture beads pre-treated with a bead blocking agent, each of said capture beads having at least one transport probe affixed thereto;

    preparing a plurality of reporter beads pre-treated with the bead blocking agent, each of said reporter beads having at least one signal probe affixed thereto;

    mixing said capture beads and said reporter beads under binding conditions so as to permit formation of a dual bead complex if said target agent is present in the sample, the reporter bead and capture bead each being bound to the target agent;

    isolating the dual bead complex from the mixture to obtain an isolate;

    exposing the isolate to a capture field on an optical bio-disc, the capture field having a capture agent that binds to the dual bead complex; and

    detecting the presence of the dual bead complex in the disc to indicate that the target agent is present in the sample.

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