Spotting device for fabricating microarrays of biological samples and spotting pin inserted therein

• US 20020176805A1
• Filed: 04/23/2002
• Published: 11/28/2002
• Est. Priority Date: 04/23/2001
• Status: Active Grant

• Current Assignee: Bioneer Corporation
• Original Assignee: Bioneer Corporation
• Inventors: Hang-Rae, Kim, Jung-Wook, Ahn, Han-Oh, Park, Gu-Young, Song, Il-Kyu, Choi, Yun-Seok, Cho, Gui-Hwan, Oh
• Granted Patent: US 6,953,552 B2
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0255 characterized by the form or material of the pin tip
• CPC Class Codes: B01L 9/00 Supporting devices; Holding devices
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

The present invention relates to a spotting device for fabricating microarrays of biological materials and a spotting pin inserted therein. More particularly, the present invention is directed to a spotting device, which comprises several pins and pin-guiding holder that allows the pins to move accurately and rectilinearly toward up-down direction by guiding and supporting pins at several points, and spotting pins inserted therein.

Claims

1. A spotting device for fabricating microarrays of biological samples, which comprises:

a plurality of spotting pins for spotting biological samples on a plate;

an upper guiding plate which has pin-inserting holes to form at least two (2) pin-supporting points;

a guide wire which forms at least one (1) pin-supporting point;

a regulation block wherein said guide wires are mounted in a plurality of up-down rows;

a spring plate which pushes said regulation block inserted in the frame toward a first side;

a regulation screw which pushes said regulation block toward a side opposed said first side;

a frame wherein said regulation block is inserted, and wherein said spring plate is inserted, and wherein said regulation screw is mounted; and

a lower guiding plate which has a plurality of pin-inserting holes to form at least two pin-supporting points.

2. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said guide wire is made of metal.

3. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said pin is selected from the group consisting of a capillary pin and a stealth pin.

4. The spotting device for fabricating microarrays of biological materials according to claim 3, wherein said capillary pin comprises a capillary part and a reservoir part connected to said capillary part, said reservoir part having larger inner diameter than said capillary part.

5. The spotting device for fabricating microarrays of biological materials according to claim 4, wherein the inner diameter of said capillary part is between 0.03 times to 0.2 times that of the reservoir part.

6. The spotting device for fabricating microarrays of biological materials according to claim 4, wherein the inner diameter of said capillary part is between 0.01 mm to 0.5 mm and the length said capillary is 0.5 mm to 3 mm.

7. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said guide wire is inserted into respective pairs of holes formed in upper and lower rows on the regulation block.

8. The spotting device for fabricating microarrays of biological materials according to claim 1, wherein said pins are inserted into the spotting device while being held in a stopper.

9. The spotting device for fabricating microarrays of biological materials according to claim 8, wherein said stopper is made of an elastic material.

21. The spotting device for fabricating microarrays of biological materials according to claim 3, wherein said pins are inserted into the spotting device while being held in a stopper.

10. A spotting pin for fabricating microarrays of biological samples, comprising a capillary part and a reservoir part connected on said capillary part, having an inner diameter of said reservoir part that is larger than an inner diameter formed within said capillary part.

11. The spotting pin for fabricating microarrays of biological samples according to claim 10, wherein the inner diameter of said reservoir part is 0.033 times to 0.2 times that of the capillary part.

12. The spotting pin for fabricating microarrays of biological materials according to claim 10, wherein the inner diameter of said capillary part is between 0.01 mm to 0.5 mm inclusive, and the length of said capillary part is between 0.5 mm to 3 mm inclusive.

13. The spotting pin for fabricating microarrays of biological materials according to claim 12, wherein the inner diameter of said capillary part is 0.003 times to 0.2 that of the reservoir part.

14. The spotting pin for fabricating microarrays of biological materials according to claim 12, wherein the inner diameter of said capillary part is 0.003 times to 0.1 times that of the reservoir part.

15. The spotting pin for fabricating microarrays of biological materials according to claim 12, wherein the inner diameter of said capillary part is 0.003 times to 0.02 times that of the reservoir part.

16. The spotting pin for fabricating microarrays of biological materials according to claim 10, wherein the inner diameter of a tip of said capillary part is between 10 mm and 50 mm.

17. The spotting pin for fabricating microarrays of biological materials according to claim 12, wherein the inner diameter of a tip of said capillary part is between 10 mm and 50 mm.

18. The spotting pin for fabricating microarrays of biological materials according to claim 10, which is made of glass.

19. The spotting pin for fabricating microarrays of biological materials according to claim 18, which is made of Soda lime, glass or Borosilicate glass.

20. The spotting pin for fabricating microarrays of biological materials according to claim 10, characterized in that the inner shape of a capillary part is tapered so that the inner diameter of lower part is smaller than that of upper part.