Devices and methods for biochip multiplexing

• US 20020177135A1
• Filed: 07/11/2001
• Published: 11/28/2002
• Est. Priority Date: 07/27/1999
• Status: Abandoned Application

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Olsen, Gary T., Doung, Hau H., Swami, Nathan, Terbrueggen, Robert H., Blackburn, Gary, Kayyem, Jon F., Pietri, Robert, OʼConnor, Stephen D.
• Application Number:
  US09/904,175
• Publication Number:
  US 20020177135A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/3033 using heat to mix or move the fluids
• CPC Class Codes: B01F 33/403 for mixing liquids B01F33/402, B01F33/405, B01F33/406 take precedence
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/024 Storing results with means integrated into the container
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1883 using thermal insulation
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0611 duck bill valves
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 2400/0661 : shape memory polymer valves
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 : with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 : with physical movement of samples between temperature zones
• CPC Class Codes: B82Y 15/00 : Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 : Manufacture or treatment of nanostructures
• CPC Class Codes: G01N 2001/021 : Correlating sampling sites with geographical information, e.g. GPS
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 2035/00326 : Analysers with modular structure
• CPC Class Codes: G01N 35/00871 : Communications between instruments or with remote terminals

Abstract

The invention is directed to devices that allow for simultaneous multiple biochip analysis. In particular, the devices are configured to hold multiple cartridges comprising biochips comprising arrays such as nucleic acid arrays, and allow for high throughput analysis of samples.

Claims

1. A biochip cartridge comprising:

a) a reaction chamber comprising;

i) a substrate comprising an array of electrodes, each comprising;

A) a self-assembled monolayer; and

B) a capture binding ligand;

II) an inlet port for the introduction of reagents; and

b) interconnects to allow the electrical connection of said electrodes to a processor.

2. A biochip cartridge according to claim 1 wherein said capture binding ligands are capture probes.

3. A biochip cartridge according to claim 1 wherein said reaction chamber further comprises a gasket to retain fluid in contact with said array.

4. A biochip cartridge according to claim 1 further comprising a seal on said inlet port.

5. A biochip cartridge according to claim 1 wherein said reaction chamber further comprises an outlet port.

6. A biochip cartridge according to claim 1 wherein said reaction chamber is configured to minimize the introduction or retention of air bubbles upon introduction of a sample.

7. A biochip cartridge according to claim 1 wherein said array is on one surface of said substrate.

8. A biochip cartridge according to claim 1 wherein two surfaces of said substrate each comprise an array.

9. A biochip cartridge according to claim 1 further comprising a cap comprising at least one storage well comprising assay reagents.

10. A device for multiple biochip analysis comprising at least two stations configured to receive a biochip cartridge.

11. A device according to claim 10 wherein at least one station comprises a thermocontroller.

12. A device according to claim 10 wherein every station comprises a thermocontroller.

13. A device according to claim 10 wherein at least one station comprises a signalling system.

14. A device according to claim 10 wherein at least one station comprises a memory chip reader.

15. A device according to claim 10 wherein at least one station comprises a barcode reader.

16. A device according to claim 10 wherein at least one station comprises a memory chip writer.

17. A device according to claim 10 wherein at least one station comprises an encryption system.

18. A device according to claim 10 further comprising at least two biochip cartridges.

19. A device according to claim 10 further comprising a liquid handling system.

20. A device according to claim 10 further comprising a wireless communication component.

21. A device according to claim 10 further comprising a device board.

22. A device according to claim 10 further comprising a processor.

23. A device according to claim 10 further comprising a global positioning system.