Detection and/or quantification method of a target molecule by a binding with a capture molecule fixed on the surface of a disc

• US 20020177144A1
• Filed: 12/27/2001
• Published: 11/28/2002
• Est. Priority Date: 12/30/1997
• Status: Abandoned Application

• Current Assignee: Eppendorf Array Technologies SA (Eppendorf Se)
• Original Assignee: Eppendorf Array Technologies SA (Eppendorf Se)
• Inventors: Remacle, Jose, Alexandre, Isabelle, Houbion, Yves
• Application Number:
  US10/035,822
• Publication Number:
  US 20020177144A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00547 Bar codes
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00662 : Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00691 : using robots
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00704 : integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B01J 2219/0074 : Biological products
• CPC Class Codes: B01L 2200/12 : Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0806 : Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0861 : Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/545 : for laboratory containers
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6834 : Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 : using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2565/625 : being a nucleic acid test strip device, e.g. dipsticks, strips, tapes, CD plates
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 35/00069 : whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

The present invention is related to a method for the detection and/or the quantification of a target molecule by its binding with a non-cleavable capture molecule fixed on the surface of a disc comprising registered data.

The present invention is also related to a disc having fixed upon its surface a non-cleavable capture molecule, to its preparation process, and to a diagnostic and/or reading device of said disc or comprising said disc.

Claims

1. A method for the detection, identification and/or the quantification of one or more target molecule(s) present in a sample comprising the steps of :

binding said target molecule to a capture molecule bound upon the surface of a solid support, said solid support being a disc comprising registered data, said binding resulting in a signal,

detecting and/or quantifying said signal with the proviso that said signal is not obtained through cleavage of the capture molecule.

2. The method according to claim 1, wherein the signal for the detection, identification and/or quantification of the target molecule is present or read on areas of the disc different from areas wherein the registered data are present or read.

3. The method according to claim 2, wherein the signal for the detection, identification and/or quantification of the target molecule is present or read on an area being on one side of the disc, and wherein the signal of the registered data is present or read on an area being on the opposite side of the said disc.

4. The method according to claim 1, wherein the detection, identification and/or quantification is performed on areas of the disc comprising registered data.

5. The method according to claim 1, wherein the capture molecules are bound on determined locations on the disc surface according to an array having a density of at least 5 different bound capture molecules/cm² disc surface.

6. The method according to claim 1, comprising the detection, identification and/or quantification of expression of multiple genes.

7. The method according to claim 5, wherein the detection, identification and/or quantification are performed on at least 3 different arrays present upon the same disc surface.

8. The method according to claim 1, further comprising the step of reading and/or recording the registered data by a reader other than the detector used for the detection, identification and/or quantification of the signal resulting from the binding between the target molecule(s).

9. The method according to claim 1, wherein the capture molecules and the target molecules are nucleotide sequences.

10. The method according to claim 1, wherein the capture molecules and target molecules are respectively either antigens and antibodies or antibodies and antigens.

11. The method according to claim 1, wherein the capture and target molecules are respectively either receptors and ligands of said receptors or ligands and their receptors.

12. The method according to claim 1, wherein the capture molecule is a single or double stranded nucleotide sequence and wherein the target molecule is a ligand of said sequence.

13. The method according to claim 1, wherein the target molecules comprise two binding sites, one binding site to its corresponding capture molecule and another binding site for another molecule.

14. The method according to claim 13, wherein said target molecule is an enzyme.

15. The method according to claim 1, wherein the capture molecules are obtained from a chemical or biological library.

16. The method according to claim 1, wherein the capture molecules are bound to the surface of the disc and are in solution in one or more wells of multiwell plates disposed upon said surface, said solution being withdrawn and delivered on the surface in defined locations.

17. The method according to claim 5, wherein the detection of the signal allows for characterization of a chemical compound bound to its corresponding capture molecule at a determined location as a potential drug acting upon a receptor being its capture molecule.

18. The method according to claim 4, wherein the detection of the signal allows for characterization of a chemical compound bound at a determined location to a receptor as a potential drug.

19. The method according to claim 5, wherein the detection of the signal allows for characterization of a chemical compound which acts as agonist or antagonist of the fixation of a ligand to its receptor.

20. The method according to claim 5, wherein the signal observed in a determined location is correlated with the identification and/or a quantification of said target compounds.

21. The method according to claim 1, wherein the detection and/or the quantification of the signal is obtained by reflection, absorption or diffraction of a light beam, or variation of an electromagnetic field.

22. The method according to claim 1, wherein the detection and/or the quantification of the signal is made on a turning disc.

23. The method according to claim 1, wherein the detection and/or the quantification of the signal is obtained by a fluorescent light emission after excitation of the bound target and capture molecules by a light beam.

24. The method according to claim 1, wherein the detection and/or the quantification of the signal is obtained by an emission selected from the group consisting of a light beam, a radiation emission, and a magnetic field.

25. The method according to claim 24, wherein the emission of a light beam is generated by a marker bound to the target and/or the capture molecule, said marker being selected from the group consisting of chemical, biological, fluoroescent, electroluminescent and radioactive markers.

26. The method according to claim 1, wherein the signal generated by the binding between the target and the capture molecules is either a precipitate on the surface of the disc or a corrosion of one or more layer(s) of the surface of the disc.

27. The method according to claim 26, wherein the precipitate is either an opaque precipitate or a magnetic precipitate.

28. The method according to claim 26, wherein the precipitate is a deposit of a colloidal metal reagent.

29. The method according to claim 26, wherein the precipitate is a silver precipitate.

30. The method according to claim 1, wherein the binding between the target and the capture molecules allows the binding of one or more additional molecule(s) upon the target and/or the capture molecules.

31. The method according to claim 30, wherein said additional molecule is either a microbead or a magnetic particle.

32. The method according to claim 1, wherein the registered data of the disc are binary data.

33. The method according to claim 1, wherein the disc is a compact-disc.

34. The method according to claim 7, wherein reading registered data allows for the interpretation of the signal resulting from the binding between the capture and the target molecules.

35.he. The method according to claim 1, wherein reading of the registered data provides information regarding the location of the deposit of capture molecules upon the disc surface.

36. The method according to claim 2, wherein the disc has at least one portion containing a layer for writing numeric information.

37. The method according to claim 2, wherein the result data of identification and/or quantification of the bound target molecule are written on the disc as numeric data.

38. The method according to claim 2, wherein resulting data of identification and/or quantification of the bound target molecules are transformed into numeric data.

39. The method according to claim 2, wherein capture molecules are specific molecules corresponding to a limited number of data bytes.

40. The method according to the claim 39, wherein the capture molecules serve as target molecules.

41. The method according to claim 1, wherein numeric information are bytes converted into a nucleotide sequence(s) being deposited and/or present on the disc.

42. The method according to the claim 39, wherein the alignment of capture molecules is converted into information selected from the group consisting of words, numbers, music, software, and data.

43. The method according to claim 39, comprising data bytes present on the same disc as both numeric information and capture molecules.

44. The method according to claim 1, which further comprises the step of putting into fluid contact one or more chambers located upon the surface of the disc by connecting them by micro-channels and valves.

46. The disc according to claim 45, wherein the non-cleavable capture molecules and/or the target molecules are selected from the group consisting of nucleotide sequences, antigens, antibodies, receptors, ligands of receptors, receptor and enzyme peptides, lipids, saccharides, haptens, fluorophores, chromophores, catalysts, and new macromolecules obtained by combinatorial chemistry, or a combination thereof.

47. The disc according to claim 45, wherein the registered data of the disc are binary data.

48. The disc according to claim 45, wherein the registered data are grooved binary data.

49. The disc according to claim 45, which is a compact-disc.

50. The disc according to claim 45, which comprises microchannels and chambers which are connected and in fluidic contact by said microchannels.

51. The disc according to claim 45, wherein the capture molecules are bound upon the disc surface in locations being separated from areas comprising readable registered data.

52. The disc according to claim 45, wherein the registered data contain information regarding the location of the samples and/or the capture molecules on the disc surface.

53. The disc according to claim 45, wherein the capture molecules on the disc surface are a library of molecules.

54. The disc according to claim 45, wherein the capture molecules are bound upon one side of said disc, and wherein the opposite side of said disc comprises the registered data.

55. The disc according to claim 45, wherein the capture molecules are disposed on the surface of the disc as arrays of at least 5 spots per cm²/surface.

56. The disc according to claim 45, comprising at least 3 arrays for the analysis of at least 3 samples.

57. The disc according to claim 45, which comprises on its surface a polymer layer with aldehyde groups for a covalent binding of the capture molecules.

58. The disc according to claim 45, which comprises on its surface a polymer layer with acrylate groups for a covalent binding of the capture molecules.

59. The disc according to claim 45, which comprises a polymer layer which has been oxidized prior to the binding of the capture molecules.

60. The disc according to claim 45, which comprises at least at some specific locations upon its surface one or more non-fluorescent layers.

61. The disc according to claim 45, comprising a central portion being a mini-CD.

62. The disc according to claim 45, which comprises one portion containing a layer for writing numeric information.

63. The disc according to claim 51, wherein said portion is a writing mini-CD.

64. The disc according to claim 63, wherein said portion comprises data regarding location, detection, identification and/or quantification of a bound target molecule, said data being stored on the disc surface as numeric data.

65. The disc according to claim 45 wherein the capture molecules are specific and correspond to data bytes.

66. The disc according to claim 65, wherein the data bytes corresponding to capture molecules are present in line on the disc surface.

67. The disc according to claim 66, wherein the alignment of capture molecules is converted into digital information selected from the group consisting of words, numbers, music, software and data bases.

68. The disc according to claim 65, which comprises data bytes present on the same CD as both numeric and capture molecules.

69. The disc according to claim 45, which comprises molded support in contact with the disc, wherein said support divides the disc into incubation chambers

70. A method of making the disc according to claim 45, which comprises the step of binding upon the surface of a disc comprising registered data, non-cleavable capture molecules through a photoactivation of said capture molecules.

71. The method according to claim 70, wherein the binding of the non-cleavable capture molecules is obtained through a covalent link between an extremity of the capture molecule and the surface layer of the disc.

72. The method according to claim 70, wherein the disc surface is recovered by a protective layer, which allows or improves the protection and stabilization of the non-cleavable capture molecule and/or the protection, stabilization and/or detection of the binding between the target molecule and its non-cleavable capture molecule.

73. A diagnostic kit comprising the disc of the claim 45 and reactants allowing the binding between a target molecule and its capture molecule and possibly reactants allowing the detection of a signal which results from said binding.

74. A detection and/or reading device which allows the detection and/or the quantification of a signal which results from the binding between a target molecule present in a sample and its capture molecule, and which comprises the disc according to claim 45 and means for the detection and/or quantification of said signal.

75. The detection and/or reading device according to claim 74, being a compact-disc reading device.

76. The detection and/or reading device according to claim 74, which comprises a first reading head for the reading of registered data and a second reading head for the detection and/or the quantification of the signal which results from the binding between target molecule and its capture molecule.

77. The detection and/or reading device according to claim 74, comprising a disc reading means, and two separate detectors, the first detector for reading and/or following the registered data and the second detector for detecting and/or quantifying the signal resulting from the binding of the target molecule and the capture molecule.

78. The device according to claim 77, wherein the second detector further comprises means for emitting a laser beam and a light photomultiplier system.

79. The device according to claim 74, which comprises means for performing a lateral movement and a circular movement of the disc or the detector, the combination of said two movements allowing the scanning of the surface of the disc wherein the capture molecules were bound to the target molecules to be detected and/or quantified.

80. The device according to claim 74, which comprises means for lateral movement and circular movement of the disc or the detection(s), the combination of said two movements allowing the scanning of the surface of the disc wherein non-cleavable capture molecules where bound to target molecules to be detected and/or quantified.

81. A handling device for the disc according to claim 45 comprising means and media for purification of the target molecule, retro-transcription of said target molecule being a RNA sequence into a DNA sequence, a specific cleavage of said target molecule, or a genetic amplification of said target molecule.

82. The handling device according to claim 81, wherein said means comprises several chambers connected to each other by microchannels and valves.

83. The handling device according to claim 82, wherein one or more of said valves are opened by using centrifugal force of the turning disc or are opened by melting a closing material present in said valves or microchannels, either by application of a local heated material or air or by steady illumination of a laser beam upon said material.

84. The handling device according to claim 81, further comprising a heating system.

85. An apparatus for performing the method according to claim 44, comprising a handling device for a disc platform and a supporting contact with the surface of the disc allowing the formation of a series of chambers connected by microchannels upon the array, said chambers comprising capture molecules.

86. A diagnostic kit according to claim 73 further comprising reactants allowing the detection of a signal which results from said binding.

45. A disc comprising registered data, and bound upon its surface, one or more non-cleavable capture molecule(s) which allow for binding with one or more target molecule(s) to be detected, identified and/or quantified.