Compounds for the treatment of sexual dysfunction
First Claim
1. An isolated and/or purified polynucleotide comprising:
- (a) a polynucleotide encoding the polypeptide of SEQ ID NO;
2;
(b) the coding sequence of a polynucleotide of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or (c) a polynucleotide encoding the polypeptide expressed by the DNA contained in NCIMB 41110.
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Accused Products
Abstract
Polynucleotide and polypeptide sequences are described. The polypeptide sequences comprise one or more of: (a) a polypeptide having the deduced amino acid sequence translated from the polynucleotide sequence in SEQ ID NO: 1 or SEQ ID NO: 5 and variants, fragments, homologues, analogues and derivatives thereof; (b) a polypeptide of SEQ ID NO: 2 and variants, fragments, homologues, analogues and derivatives thereof; (c) a polypeptide encoded by the cDNA of NCIMB 41110 and variants, fragments, homologues, analogues and derivatives thereof; or (d) a polypeptide which has at least 78% identity to (i) the polypeptide encoded by the polynucleotide of SEQ ID NO: 1 or SEQ ID NO: 5, (ii) the polypeptide of SEQ ID NO: 2, or (iii) the polypeptide encoded by the cDNA of NCIMB 41110. Such polypeptide sequences are, inter alia, useful in the prophylaxis and/or treatment of sexual dysfunction, in particular male erectile dysfunction (MED) or female sexual dysfunction (FSD), preferably female sexual arousal disorder (FSAD).
17 Citations
23 Claims
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1. An isolated and/or purified polynucleotide comprising:
-
(a) a polynucleotide encoding the polypeptide of SEQ ID NO;
2;
(b) the coding sequence of a polynucleotide of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or(c) a polynucleotide encoding the polypeptide expressed by the DNA contained in NCIMB 41110.
-
-
2. An isolated and/or purified polynucleotide comprising:
-
(a) a nucleotide sequence that has at least 83% identity to the polynucleotide of SEQ ID NO;
1 or 5;
(b) a polynucleotide sequence encoding a polypeptide at least 78% identical to SEQ ID NO;
2; and
/or(c) a nucleotide sequence which is capable of hybridising to the coding sequence of the polynucleotide of SEQ ID NO;
1 or 5 under conditions of high stringency;
wherein said polynucleotide encodes a human soluble secreted endopeptidase (SEP).
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3. A process for producing a polypeptide comprising culturing a host cell transformed or transfected with a vector comprising a polynucleotide encoding the polypeptide of SEQ ID NO:
- 2, the coding sequence of the polynucleotide of SEQ ID NO;
1 or SEQ ID NO;
5, or a polynucleotide encoding the polypeptide expressed by the DNA contained in NCIMB 41110 under conditions sufficient for the expression of said polypeptide.
- 2, the coding sequence of the polynucleotide of SEQ ID NO;
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4. An isolated and/or purified polypeptide comprising:
-
(a) SEQ ID NO;
2;
(b) an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or(c) a polypeptide encoded by the cDNA of NCIMB 41110.
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5. An isolated and/or purified polypeptide comprising an amino acid sequence having at least 78% identity to SEQ ID NO:
- 2, wherein said polypeptide has SEP activity.
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6. An antibody against a polypeptide comprising:
-
(a) SEQ ID NO;
2;
(b) an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or(c) a polypeptide encoded by the cDNA of NCIMB 41110.
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7. A method for the treatment of sexual dysfunction in a patient comprising administering to the patient a therapeutically effective amount of an anti-SEP polypeptide antibody or a compound that inhibits a SEP polypeptide, wherein said SEP polypeptide comprises:
-
(a) SEQ ID NO;
2;
(b) an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or(c) a polypeptide encoded by the cDNA of NCIMB 41110. - View Dependent Claims (8, 9)
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10. Cells genetically engineered ex vivo or in vivo to overexpress or underexpress a SEP polypeptide comprising:
-
(a) SEQ ID NO;
2;
(b) an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO;
1 or SEQ ID NO;
5; and
/or(c) a polypeptide encoded by the cDNA of NCIMB 41110. - View Dependent Claims (11, 13, 14, 15, 16, 17, 18, 19, 20)
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12. A method for identifying an agent which binds to and/or modulates a SEP polypeptide comprising contacting said agent with a polypeptide comprising:
- SEQ ID NO;
2, an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO;
1 or SEQ ID NO;
5;
or a polypeptide encoded by the cDNA of NCIMB 41110, and determining whether binding and/or modulation occurs.
- SEQ ID NO;
-
21. A method for identifying an agent which inhibits a peptidase comprising contacting said peptidase and a peptidase substrate peptide selected from the group consisting of Rhodamine green-Gly-Gly-dPhe-Leu-Arg-Arg-Val-Cys(QSY™
- 7)-β
Ala-NH2, 5-(and
6) tetramethyl rhodamine Gly-Gly-dPhe-Leu-Arg-Arg-Val-Cys(QSY™
7)-β
Ala-NH2, and 5-carboxyfluorescein-Gly-Gly-dPhe-Leu-Arg-Arg-Val-Cys (5-(and
6) tetramethylrhodamine)-β
Ala-NH2, in the presence of said agent, wherein said substrate peptide is capable of providing a detectable signal in response to cleavage by said peptidase as detected by Fluorescence Resonance Energy Transfer (FRET) assay, wherein said agent is identified as an inhibitor of said SEP polypeptide if there is a decrease in the detectable signal in the presence of said agent as compared to in the absence of said agent. - View Dependent Claims (22, 23)
- 7)-β
Specification