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Determining protein function in cell culture using RNA interference

  • US 20020182590A1
  • Filed: 05/24/2002
  • Published: 12/05/2002
  • Est. Priority Date: 05/25/2001
  • Status: Abandoned Application
First Claim
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1. A method for assigning functional properties to polypeptides comprising:

  • (a) obtaining a population of double-stranded (ds) RNA molecules, where each unique dsRNA molecule is segregated from other members of the dsRNA population;

    (b) contacting one or more members of said dsRNA population with a host cell; and

    (c) measuring one or more phenotypic parameters of the host cell of step (b), wherein a change in a phenotypic parameter in the host cell of step (b), as compared to a host cell not contacted with dsRNA, assigns to a polypeptide or polypeptides encoded by the one or more members of said dsRNA population of step (b) a functional property.

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