Method for assaying biological and other constituents using synthetic nucleounits in lateral flow, liquid, and dry chemistry techniques
First Claim
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1. A method for detecting an analyte of interest using nucleounits targeted to said analyte of interest comprising:
- a) identifying a nucleounit from a mixture of synthetic random sequences of nucleounit libraries, by the method comprising i) contacting the analyte of interest with said mixture, wherein the nucleounits have an affinity to the analyte of interest and bind the said analyte;
ii) the unbound nucleounits are removed by partitioning;
iii) the remaining nucleounits are amplified by polymerase chain reaction to obtain an enriched solution of nucleounits with high affinity for the analyte of interest;
b) nucleounits are then conjugated to indicator for the analyte of interest forming an nucleounit indicator conjugate;
c) detecting the analyte of interest using said nucleounit indicator conjugate in a buffer.
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Abstract
The present invention is a method for the use of particles made up of nucleotides or fragments of base groups of DNA and RNA molecules herein referred to as synthetic nucleounits which can be used as recognition molecules with specificity and sensitivity significantly greater than that of antibodies which are used in clinical diagnostics, biotechnology, and research.
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7 Claims
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1. A method for detecting an analyte of interest using nucleounits targeted to said analyte of interest comprising:
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a) identifying a nucleounit from a mixture of synthetic random sequences of nucleounit libraries, by the method comprising i) contacting the analyte of interest with said mixture, wherein the nucleounits have an affinity to the analyte of interest and bind the said analyte;
ii) the unbound nucleounits are removed by partitioning;
iii) the remaining nucleounits are amplified by polymerase chain reaction to obtain an enriched solution of nucleounits with high affinity for the analyte of interest;
b) nucleounits are then conjugated to indicator for the analyte of interest forming an nucleounit indicator conjugate;
c) detecting the analyte of interest using said nucleounit indicator conjugate in a buffer. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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