Device and methods for detecting samples in a flow cytometer independent of variations in fluorescence polarization
First Claim
1. A sample detection apparatus, comprising a polarized radiation source, flow chamber and signal detector, said flow chamber placed to contact polarized radiation from said polarized radiation source, said signal detector placed to selectively detect radiation propagated from said flow chamber at about 54.7 degrees from the direction of polarization of said contacted polarized radiation.
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Abstract
The invention provides a sample detection apparatus, including a polarized radiation source, flow chamber and signal detector, the flow chamber placed to contact polarized radiation from the polarized radiation source, the signal detector is placed to selectively detect radiation propagated from the flow chamber at about 54.7 degrees from the direction of polarization of the contacted polarized radiation. Also provided is a method of detecting fluorescent intensity for a sample in a flow cytometer independent of anisotropic radiation emission. The method includes the steps of: (a) contacting a sample in a flow cytometer with polarized radiation; and (b) detecting radiation emitted by the sample at about 54.7 degrees with respect to the direction of polarization of the polarized radiation at the point of sample contact.
54 Citations
19 Claims
- 1. A sample detection apparatus, comprising a polarized radiation source, flow chamber and signal detector, said flow chamber placed to contact polarized radiation from said polarized radiation source, said signal detector placed to selectively detect radiation propagated from said flow chamber at about 54.7 degrees from the direction of polarization of said contacted polarized radiation.
- 6. A flow cytometer, comprising a polarized radiation source, flow chamber and signal detector, said flow chamber placed to contact polarized radiation from said polarized radiation source, said signal detector placed to selectively detect radiation propagated from said flow chamber at about 54.7 degrees from the direction of polarization of said contacted polarized radiation.
- 11. A sample detection apparatus, comprising a polarized radiation source, flow chamber, signal detector and a trajectory of a sample stream that is orthogonal to said polarized radiation source and said signal detector, said polarized radiation source placed to contact said trajectory of said sample stream with polarized radiation, said polarization direction being 35.3 degrees from a line parallel to said trajectory of said sample stream.
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12. A flow cytometer, comprising a polarized radiation source, flow chamber, signal detector and a trajectory of a sample stream that is orthogonal to said polarized radiation source and said signal detector, said polarized radiation source placed to contact said trajectory of said sample stream with polarized radiation, said polarization direction being 35.3 degrees from a line parallel to said trajectory of said sample stream.
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13. A method of detecting fluorescent intensity for a sample in a flow cytometer independent of anisotropic radiation emission, comprising:
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(a) contacting a sample in a flow cytometer with polarized radiation; and
(b) detecting radiation emitted by said sample at about 54.7 degrees with respect to the direction of polarization of said polarized radiation at the point of sample contact.
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19. A method for determining scattered irradiation passing through a filter, comprising determining a degree of polarization through a filter, wherein said degree of polarization is greater than 0.5.
Specification