Process for labeling a ribonucleic acid
First Claim
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1. A process for labeling a synthetic or natural ribonucleic acid (RNA), comprising:
- obtaining prepared RNA by a method selected from the group consisting of;
amplifying the RNA;
amplifying a template deoxyribonucleic acid (DNA) into the RNA; and
amplifying a template DNA of the RNA and transcribing the resulting amplified DNA into the RNA;
fragmenting the prepared RNA to produce a plurality of RNA fragments having freed terminal phosphates located at at least one of the 3′ and
5′
ends of the RNA fragments; and
labeling a plurality of the RNA fragments at freed terminal phosphates.
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Abstract
The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a terminal phosphate of each fragment for further reaction, and labeling each fragment at the freed terminal phosphate which is located at the 3′ end and/or the 5′ end of each fragment of the RNA, and to the use of such RNA fragments, for example, in the field of medical diagnosis.
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Citations
20 Claims
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1. A process for labeling a synthetic or natural ribonucleic acid (RNA), comprising:
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obtaining prepared RNA by a method selected from the group consisting of;
amplifying the RNA;
amplifying a template deoxyribonucleic acid (DNA) into the RNA; and
amplifying a template DNA of the RNA and transcribing the resulting amplified DNA into the RNA;
fragmenting the prepared RNA to produce a plurality of RNA fragments having freed terminal phosphates located at at least one of the 3′ and
5′
ends of the RNA fragments; and
labeling a plurality of the RNA fragments at freed terminal phosphates. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 19)
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16. A process for labeling a synthetic or natural ribonucleic acid (RNA), comprising:
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obtaining extracted RNA by extraction from cell material;
fragmenting the extracted RNA to produce a plurality of RNA fragments having freed terminal phosphates at at least one of 3′ and
5′
ends of the fragments, andlabeling a plurality of the RNA fragments at freed terminal phosphates.
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18. A process for labeling a synthetic or natural ribonucleic acid (RNA), comprising:
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obtaining transcribed RNA by;
extracting a template deoxyribonucleic acid (DNA) of the RNA from cell material and transcribing the extracted DNA into RNA;
fragmenting the transcribed RNA to produce a plurality of RNA fragments having freed terminal phosphates located at at least one of the 3′ and
5′
ends of the fragments; and
labeling a plurality of the RNA fragments at freed terminal phosphates.
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20. A process for labeling a synthetic or natural ribonucleic acid (RNA), comprising:
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obtaining a template deoxyribonucleic acid (DNA) of RNA by extraction from cell material;
amplifying the DNA into the RNA;
fragmenting the amplified RNA to produce a plurality of RNA fragments having freed terminal phosphates located at at least one of the 3′ and
5′
ends of the fragments; and
labeling a plurality of the RNA fragments at freed terminal phosphates.
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Specification