Systems and methods for introducing samples into microfluidic devices
First Claim
1. A pressure-driven microfluidic separation device comprising:
- a separation channel having a first end and a second end, and containing stationary phase material; and
a sample input adapted to provide a fluidic sample to the separation channel between the first end and the second end.
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Abstract
A pressure-driven microfluidic device for separating chemical or biological species from a sample includes on-column injection, namely, a separation channel containing stationary phase material and a sample input disposed between a first end and a second end of the separation channel or column. One or many separation channels may be provided in a single microfluidic device, which may be fabricated with sandwiched stencil layers using various materials including polymers. Sealing means associated with a sample input, such as a mechanical seal adapted to selectively seal the sample input, are provide. Various sample injector configurations are provided. A separation system including a microfluidic device having on-column injection further includes a pressure source and a detector.
201 Citations
49 Claims
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1. A pressure-driven microfluidic separation device comprising:
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a separation channel having a first end and a second end, and containing stationary phase material; and
a sample input adapted to provide a fluidic sample to the separation channel between the first end and the second end. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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19. A pressure-driven microfluidic separation device comprising:
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a plurality of separation channels each having a first end and a second end; and
a plurality of sample inputs, each sample input of the plurality of sample inputs being in fluid communication with a separation channel of the plurality of separation channels and being disposed between the first end and the second end.
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37. A separation system comprising:
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a pressure-driven microfluidic separation device for separating a sample into a plurality of species, the separation device having a separation channel and a sample input, the separation channel having a first end and a second end, the sample input being adapted to supply fluid to the separation channel, and the sample input being disposed between the first end and the second end;
a pressure source adapted to supply a pressurized fluid to the separation device; and
a detector adapted to detect a property of at least one species of the plurality of species.
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49. A method for loading a sample into a pressure-driven separation channel, the method comprising the steps of:
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providing a separation channel containing a stationary phase material, the separation channel having a first end, a second end, and a sample inlet port permitting fluid communication with the separation channel between the first end and the second end;
initiating a flow of mobile phase solvent through the separation channel;
pausing the flow of mobile phase solvent;
supplying a sample to the sample inlet port; and
sealing the sample inlet port.
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Specification