Biosensor

• US 20020189941A1
• Filed: 05/15/2002
• Published: 12/19/2002
• Est. Priority Date: 11/15/1999
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Fukuoka, Takao, Hamamoto, Katsumi, Yagi, Yuji
• Granted Patent: US 6,916,410 B2
• Time in Patent Office: Days
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A biosensor is constituted by disposing an electrode system including a working electrode and a counter electrode on a substrate, forming an inorganic particulate-containing layer containing inorganic particulates thereon, and forming a reagent layer containing a reagent thereon. The inorganic particulates make it possible to prevent impurities in a sample from being in contact with the electrode system and being adsorbed therein, so that measurement is performed with high precision. The inorganic particulate-containing layer can be formed by applying a dispersion system of inorganic particulates and drying them, and it is preferable that the inorganic particulates are contained in the form of aggregates.

Claims

1. A biosensor comprising a substrate, a reagent layer containing a reagent, and an electrode system including a working electrode and a counter electrode, the electrode system being disposed on the substrate, and the reagent layer being formed on the electrode system,

wherein the reagent layer further comprises inorganic particulates.

2. The biosensor according to claim 1, wherein the reagent layer is a single layer.

3. The biosensor according to claim 1, wherein the reagent layer is a laminate comprising a reagent-containing layer containing the reagent and a particulate-containing layer containing the inorganic particulates.

4. The biosensor according to claim 3, wherein the reagent-containing layer is formed on the electrode system via the inorganic particulate-containing layer.

5. The biosensor according to any one of claims 1 to 4, wherein the reagent layer contains aggregates of inorganic particulates.

6. The biosensor according to any one of claims 1 to 4, wherein a layer containing inorganic particulates is formed by applying a dispersion system in which the inorganic particulates are dispersed on the electrodes, and drying the dispersion system.

7. The biosensor according to any one of claims 1 to 4, wherein the inorganic particulates are expansive particulates.

8. The biosensor according to any one of claims 1 to 4, wherein the inorganic particulates are particulates of clay mineral.

9. The biosensor according to claim 8, wherein the clay mineral is expansive phyllosilicate.

10. The biosensor according to claim 9, wherein the expansive phyllosilicate is at least one of smectite and expansive mica.

11. The biosensor according to claim 10, wherein the smectite is at least one selected from the group consisting of hectorite, saponite, and montmorillonite.

12. The biosensor according to claim 10, wherein the expansive mica is at least one of sodium tetrasilicon fluoride mica and teniorite.

13. The biosensor according to any one of claims 1 to 4, wherein the content of the inorganic particulates in the layer containing inorganic particulates is in a range from 0.14 to 14 mg per cm² of an area.

14. The biosensor according to any one of claims 1 to 4, wherein the thickness of the layer containing inorganic particulates is in a range from 0.05 to 5 μ

m.

15. The biosensor according to any one of claims 1 to 4, wherein the reagent layer further comprises particulates of a water-insoluble high molecular weight compound.

16. The biosensor according to any one of claims 1 to 4, wherein a layer containing particulates of a water-insoluble high molecular weight compound is formed on the electrode system via the reagent layer.

17. The biosensor according to claim 15 or 16, wherein the water-insoluble high molecular weight compound is a polymer or a copolymer containing at least one of acrylic acid, methacrylic acid, maleic acid, acrylic ester, methacrylic acid ester, maleic acid ester, styrene, and styrene derivative monomer, or a polyamide-based high molecular weight compound.

18. The biosensor according to claim 15 or 16, wherein the average particle diameter of the particulates of the water-insoluble high molecular weight compound is in a range from 0.1 to 45 μ

m.

19. The biosensor according to any one of claims 1 to 4, wherein a surfactant-containing layer containing a surfactant further is formed on the reagent layer.

20. The biosensor according to any one of claims 1 to 4, wherein the electrodes are at least one selected from the group consisting of gold electrodes, carbon electrodes and silver electrodes.

21. The biosensor according to any one of claims 1 to 4, wherein the reagent contains a redox enzyme.

22. The biosensor according to any one of claims 1 to 4, wherein the reagent contains a redox enzyme and an electron acceptor in a reaction of the enzyme.

23. The biosensor according to any one of claims 1 to 4, wherein the sample to be measured is blood.