Microfluidic injection and separation system and method

• US 20020189946A1
• Filed: 03/29/2002
• Published: 12/19/2002
• Est. Priority Date: 02/11/2000
• Status: Abandoned Application

• Current Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Original Assignee: Aclara Biosciences, Inc (Laboratory Corporation Of America Holdings)
• Inventors: Wainright, Ann K., Williams, Stephen J.
• Application Number:
  US10/113,170
• Publication Number:
  US 20020189946A1
• Time in Patent Office: Days
• US Class Current: 204/453
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: C07K 1/28 Isoelectric focusing
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

Methods of sample loading and separation in a microfluidics device are described. The methods provide high resolution and high signal intensity, using, in a preferred embodiment, a simple two-electrode injection scheme with isotachophoretic (ITP) stacking, followed by ZE separation in the same channel.

Claims

1. A method for injecting a sample comprising a plurality of charged components and separating the components by electrophoresis in a microfluidics device,

wherein said microfluidics device includes:

a separation channel, having an upstream junction at which first and second channels intersect, said first and second channels terminating in first and second reservoirs, designated T and S/D, respectively;

a first side channel, intersecting said separation channel downstream of said junction, and terminating in a first side reservoir, designated D/S;

a second side channel intersecting one of said separation channel, first channel, and second channel, and terminating in a second side reservoir, designated L;

an outlet reservoir at a downstream terminus of said separation channel; and

, for each said reservoir, an electrode in fluid contact with the reservoir;

the method comprising;

(a) placing into said channels and reservoirs, a leading electrolyte solution, comprising an ion with higher mobility in an electric field than any of said charged sample components;

(b) placing into one of said S/D reservoir and said D/S reservoir, the sample solution, and placing into said T reservoir, a terminating electrolyte solution, comprising an ion with lower mobility in an electric field than any of said charged sample components;

(c) creating a voltage gradient between the S/D reservoir and the D/S reservoir, such that the sample solution migrates into a sample-loading region of the separation channel, between said upstream junction and said first side channel;

(d) creating a voltage gradient between the T reservoir and the S/D reservoir, such that the terminating electrolyte solution migrates through the first channel, to an upstream boundary of the sample solution in the sample-loading region, and into the second channel;

(e) creating a voltage gradient between the T reservoir and the outlet reservoir, such that the sample components become stacked within a region of the separation channel which is downstream of the second side channel; and

(f) creating a voltage gradient between the L reservoir and the outlet reservoir, such that leading electrolyte solution migrates from said second side channel and through said stacked sample components,

whereby the sample components move through the separation channel and separate into discrete bands according to their electrophoretic mobilities.

2. The method of claim 1, wherein, in steps (c)-(f), voltages are applied to the two electrodes specified, and the remaining electrodes are in a floating state.

3. The method of claim 1, wherein the sample solution is placed into reservoir S/D, and in step (c), the sample solution migrates into said separation channel region in a downstream direction.

4. The method of claim 1, wherein the sample solution is placed into reservoir D/S, and in step (c), the sample solution migrates into said separation channel region in an upstream direction.

5. The method of claim 1, wherein the second side channel intersects the separation channel, downstream of the first side channel.

6. The method of claim 1, wherein the second side channel intersects the first or second channel.

7. The method of claim 1, wherein the second side channel intersects the separation channel, at a position directly opposite the first side channel.

8. The method of claim 1, wherein the sample-loading region of the separation channel, between the upstream junction and the first side channel, is about 0.5-5 cm in length.

9. The method of claim 1, wherein the second side channel contains a leading electrolyte solution different from that used to fill the remaining channels in step (a).

10. The method of claim 1, further comprising, following step (c) and prior to step (d):

creating a voltage gradient between (i) a reservoir downstream of the first side channel and (ii) reservoir S/D, such that a desired amount of sample solution is displaced from the sample-loading region into the S/D channel.

11. The method of claim 2, wherein the electrodes are controlled by a single high voltage power source which employs multiplexed switching among the electrodes.

12. The method of claim 1, wherein the charged components are selected from the group consisting of nucleic acids, proteins, polypeptides, polysaccharides, and synthetic polymers.

13. The method of claim 1, wherein the charged components comprise labeled molecules having distinct and characterized electrophoretic mobilities, said molecules having been cleaved from molecular species with biological or chemical recognition properties in the course of a multiplexed chemical or biochemical assay.

14. The method of claim 13, wherein the sample solution further comprises a cell lysate and reagents used in said assay.

15. The method of claim 13, wherein the sample solution further comprises live cells and reagents used in said assay.

16. The method of claim 1, further comprising detecting said separated components.

17. The method of claim 16, wherein the concentration of at least one detected sample component in said sample is less than 1 pM.

18. The method of claim 1, wherein said sample is a clinical sample derived from a body fluid or tissue sample.

19. The method of claim 1, wherein said sample is from an environmental source.