Bio-tolerant substrata having analyte binding microarray
First Claim
1. An ocular device comprising a transparent substrate and a plurality of specific binding molecules bound thereto, each in a discrete location, wherein said specific binding molecules selectively bind analytes contained in ocular secretions upon contacting an ocular surface with said device.
2 Assignments
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Accused Products
Abstract
Contact lenses or similar ocular devices are disclosed that have specific binding molecule microarrays printed on or embedded in them to bind various analytes present in tears. Tear are used as a non-invasive alternative to the monitoring of proteins and other constituents found in serum, especially low molecular weight analytes and low abundance proteins. The devices can be placed in a developing reagent or the subject wearing the device can perceive changes in the visual filed that can be used to diagnose disease status as well as monitor various physiological and ambient environmental (exogenous) conditions.
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Citations
42 Claims
- 1. An ocular device comprising a transparent substrate and a plurality of specific binding molecules bound thereto, each in a discrete location, wherein said specific binding molecules selectively bind analytes contained in ocular secretions upon contacting an ocular surface with said device.
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9. A method of diagnosis using ocular secretions comprising:
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a) contacting an ocular device comprising a transparent substrate and a plurality of specific binding molecules bound thereto, each in a discrete location, with the ocular surface of a subject, wherein said specific binding molecules selectively bind analytes contained in ocular secretions upon contacting an ocular surface with said device;
b) allowing the ocular device and ocular fluids to interact for a specified period of time; and
c) determining the presence, absence or amount of at least one analyte present in said secretion that selectively binds to the specific binding molecules, wherein the presence, absence or amount of said at least one analyte correlates with a specific diagnosis. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of detecting a harmful agent using a device placed on an ocular surface comprising:
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(a) contacting an ocular device comprising a transparent substrate and a plurality of specific binding molecules bound thereto, each in a discrete location, with the ocular surface of a subject, wherein said specific binding molecules selectively bind analytes from the surrounding ambient environment, (b) allowing the ocular device and ambient environment to interact for a specified period of time; and
(c) determining the presence, absence or amount of a harmful agent in said environment that selectively binds to the specific binding molecules, wherein the presence of toxic agent binding correlates with a presence of a toxic agent in the ambient environment. - View Dependent Claims (21, 22, 23, 25, 26, 27)
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24. A method of determining the response to a vaccine comprising:
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a) contacting an ocular device comprising at least one vaccine component with an ocular surface of an immunized subject;
b) allowing the ocular device and ocular fluid to interact for a specified period of time; and
c) determining the presence of an immunoglobulin contained in said secretion that selectively binds to the vaccine component attached to the ocular device, wherein the presence of said immunoglobulin correlates with a specific response to said vaccine.
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28. A method of determining a toxic or effective response to a chemical comprising:
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a) contacting an ocular device comprising an array having a plurality of specific binding molecules with an ocular surface of a dosed subject;
b) allowing the ocular device and ocular surface to interact for a specified period of time;
c) determining the presence of an analyte comprised in said secretion that is produced in a toxic or effective response and which selectively binds to the device, wherein the presence of said toxic substance correlates with a negative response to said drug.
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29. A method of determining the response to an allergen comprising:
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a) contacting an ocular device comprising an array of allergens with an ocular surface of a subject;
b) allowing the ocular device and ocular fluid to interact for a specified period of time;
c) removing said device after specified time period;
d) determining the presence, absence or amount of IgE comprised in said secretion that selectively binds to the allergens in or on the ocular device, wherein the presence of IgE correlates with a specific response to said allergen.
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30. A method of detecting an analyte in ocular secretions comprising:
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contacting an ocular device comprising a transparent substrate and having an analyte adsorbing region for reversibly adsorbing the analyte allowing the ocular device and ocular fluids to interact for a period of time sufficient for analyte to adsorb to said ocular device removing the ocular device removing adsorbed analyte from the ocular device; and
determining the presence, absence or amount of at least one analyte present in said secretion that bound to the ocular device wherein the presence, absence or amount of said at least one analyte correlates with a specific diagnosis. - View Dependent Claims (31, 32)
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- 33. A therapeutic implantable device which is capable of detecting an analyte in body fluids comprising a therapeutic implantable device having a region which is a substrate having a plurality of specific binding molecules bound thereto, each in a discrete location, wherein said specific binding molecules selectively bind analytes contained in body fluids upon contact with said device, and wherein said region does not interfere with the therapeutic function of the device.
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38. A vessel or conduit for holding body fluids which is capable of detecting an analyte in body fluids having a region contacting said body fluids which is a substrate having a plurality of specific binding molecules bound thereto, each in a discrete location, wherein said specific binding molecules selectively bind analytes contained in said body fluids upon contact with said vessel or conduit.
Specification