Single polymer matrix unit chromatography
First Claim
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1. A method of physically separating polypeptide constituents of a sample fluid, the method comprising:
- (a) providing a sample fluid comprising a mixture of polypeptides of differing physical and/or chemical properties;
(b) contacting the fluid with at least two individual polymer matrix units, each polymer matrix unit preferentially accepting a different set of polypeptides on the basis of one or more physical and/or chemical properties;
(c) extracting the set of polypeptides from each polymer matrix unit.
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Abstract
A method and apparatus for physically separating polypeptide constituents of a sample fluid by (a) providing a sample fluid comprising a mixture of polypeptides of differing physical and/or chemical properties; (b) contacting the fluid with at least two individual polymer matrix units, each polymer matrix unit preferentially accepting a different set of polypeptides on the basis of one or more physical and/or chemical properties; and (c) extracting the set of polypeptides from each polymer matrix unit.
6 Citations
135 Claims
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1. A method of physically separating polypeptide constituents of a sample fluid, the method comprising:
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(a) providing a sample fluid comprising a mixture of polypeptides of differing physical and/or chemical properties;
(b) contacting the fluid with at least two individual polymer matrix units, each polymer matrix unit preferentially accepting a different set of polypeptides on the basis of one or more physical and/or chemical properties;
(c) extracting the set of polypeptides from each polymer matrix unit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 131, 133, 134, 135)
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84. A device for physically separating polypeptide constituents of a sample fluid, the device comprising:
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(a) at least two spatially separated polymer matrix units, each polymer matrix unit preferentially accepting a different set of polypeptides on the basis of one or more physical and/or chemical properties;
(b) a sample fluid exposing device for exposing the sample fluid in a manner which permits access by the spatially separated polymer matrix units;
(c) a substrate for receiving sample aliquots from the polymer matrix units;
(d) a mechanical movement device for mechanically contacting the polymer matrix units with the sample fluid to preferentially load each polymer matrix unit with a fraction of the sample fluid, and to contact the loaded polymer matrix units with the substrate to deposit one or more fractionated aliquots on the substrate, wherein the mechanical movement device operates to achieve its purpose by mechanically moving any one or more of;
(i) the polymer matrix units;
(ii) the sample fluid exposing device; and
(iii) the substrate. - View Dependent Claims (85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 127, 128, 129, 130, 132)
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126. A substrate comprising a one-dimensional or two dimensional array of chromatographic bead particles, said particles being divided into sets of two or more beads, wherein each set of bead particles preferentially accepts a set of polypeptides based on one or more physical and/or chemical characteristics.
Specification