Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
First Claim
1. A fluorescent compound comprising:
- a fluorophore including a charged moiety;
an anilinic group in relation to the fluorophore;
a boronate moiety capable of binding glucose;
a linker moiety that links the fluorophore to the boronate binding moiety;
wherein the compound exhibits an alteration in fluorescent properties when the boronate moiety is bound to glucose.
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Abstract
Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
68 Citations
46 Claims
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1. A fluorescent compound comprising:
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a fluorophore including a charged moiety;
an anilinic group in relation to the fluorophore;
a boronate moiety capable of binding glucose;
a linker moiety that links the fluorophore to the boronate binding moiety;
wherein the compound exhibits an alteration in fluorescent properties when the boronate moiety is bound to glucose. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 16, 38)
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- 10. A compound having a formula comprising:
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15. A fluorescent compound of the general formula:
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34. A method of quantifying the amount of glucose, the method comprising:
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interrogating a subcutaneously implantable biosensor which includes biosensor molecules contained in or attached to a polymer matrix, with a light source to produce an excited state of the biosensor molecules that yield an emission signal that can be correlated to the amount of glucose in body fluids surrounding an implanted biosensor, wherein the biosensor molecules include an anilinic group and a fluorophore that includes an iminium ion;
detecting the emission signal; and
quantifying the amount of glucose from the emission signal.
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Specification