Microfabricated separation device employing a virtual wall for interfacing fluids

• US 20020195343A1
• Filed: 01/24/2002
• Published: 12/26/2002
• Est. Priority Date: 06/20/2001
• Status: Abandoned Application

• Current Assignee: Cytonome Inc.
• Original Assignee: Coventor, Inc. (LAM Research Corporation)
• Inventors: Bohm, Sebastian, Gilbert, John
• Application Number:
  US10/057,354
• Publication Number:
  US 20020195343A1
• Time in Patent Office: Days
• US Class Current: 204/453
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/0086 Dimensions of the flow channels
• CPC Class Codes: B01J 2219/00905 Separation
• CPC Class Codes: B01J 2219/00912 by electrophoresis
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 2035/1037 Using surface tension, e.g. pins or wires
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44782 of a plurality of samples
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

A fluid interface port in a separation device for separating a sample into different components is provided. The separation device includes an array of separation channels and the fluid interface port comprises an opening formed in the side wall of a separation channel sized and dimensioned to form a virtual wall when the separation channel is filled with a separation medium. The fluid interface port is utilized to introduce a liquid sample into the separation medium. The fluid interface ports formed in the array of separation channels are organized into one or more sample injectors. A cathode reservoir is multiplexed with one or more separation channels. To complete an electrical path, an anode reservoir which is common to some or all separation channels is also provided.

Claims

1. A separation device, comprising:

one or more anode reservoirs;

a plurality of separation channels connected to said anode reservoirs, each of said separation channels having an interior bounded by a side wall;

one or more fluid interface ports formed in the side wall of one of said separation channels to provide access to the interior of the separation channel, wherein a separation medium disposed in the interior of the separation channel forms a virtual wall at the fluid interface port; and

at least one cathode reservoir multiplexed with two or more of said separation channels.

2. The device of claim 1, further comprising an electrode array coupleable to said reservoirs and said fluid interface ports.

3. The device of claim 1, wherein the device has an outer perimeter and a center and the separation channels connect the outer perimeter to the center.

4. The device of claim 1, wherein the fluid interface port has a dead volume that is less than about one nanoliter.

5. The device of claim 1, wherein the fluid interface port has zero dead volume.

6. The device of claim 1, wherein the fluid interface port comprises an array of apertures forming virtual walls.

7. The device of claim 1, wherein the fluid interface port has a diameter between about 25 μ

m and about 125 μ

m.

8. The separation device of claim 1, wherein the device is a capillary array electrophoresis plate.

9. The separation device of claim 1, wherein the device comprises an electrochromatographic system.

10. The separation device of claim 1, wherein the device comprises a pressure-driven chromatographic system.

11. The separation device of claim 1, wherein the device comprises an isoelectric focusing system.

13. The separation device of claim 12, wherein the fluid interface port has a diameter between about 25 μ

m and about 125 μ

m.

14. The separation device of claim 12, wherein the first and second substrate are made of glass.

15. The separation of claim 12, wherein the first and second substrate are made of plastic.

16. The separation device of claim 12, further comprising an electrode array coupleable to said reservoir array layer.

17. The separation device of claim 16, wherein said electrode array is integral with the two substrates.

18. The separation device of claim 17, wherein the fluid interface ports are regularly spaced on one of said substrates to receive solutions from a parallel loading device.

19. The separation device of claim 12, wherein the first substrate includes an array of electrodes aligned with the fluid interface ports, the cathode reservoirs, and the anode reservoirs to make electrical contacts with a plurality of solutions in a combination of the fluid interface ports, the cathode reservoirs, and the anode reservoirs.

20. The separation device of claim 12, wherein the separation device has H holes, and wherein H is approximately equal to the number of samples to be simultaneously processed in the separation device.

21. The separation device of claim 12, wherein the separation device is made of a combination of glass and plastic.

22. The separation device of claim 12, further comprising an electrode array in electrical contact with the separation device.

23. The separation device of claim 12, wherein a plurality of fluid interface ports are disposed in one of said separation channels.

24. The separation device of claim 12, wherein the first substrate includes an array of electrodes aligned with the fluid interface ports to make electrical contacts with a plurality of solutions in the fluid interface ports.

25. The separation device of claim 12, wherein the fluid interface port has a diameter between about 25 μ

m and about 125 μ

m.

26. The separation device of claim 12, wherein the device is a capillary array electrophoresis plate.

27. The separation device of claim 12, wherein the device comprises an electrochromatographic system.

28. The separation device of claim 12, wherein the device comprises a pressure-driven chromatographic system.

29. The separation device of claim 12, wherein the device comprises an isoelectric focusing system.

12. A separation device, comprising:

an array of microfabricated separation channels formed at a surface of a first microfabricated substrate and a corresponding surface of a second substrate bonded to the surface of said first substrate, each of said channels having an interior bounded by a side wall, a first end and a second end;

an array of fluid interface ports formed in the side walls of said separation channels to provide access to the interiors of the separation channels, wherein a separation medium disposed in the interior of the separation channel forms a virtual wall at each of the fluid interface ports in the array;

an array of cathode reservoirs connected to the first end of each of the separation channels; and

an array of anode reservoirs, wherein at least one anode reservoir is connected to the respective second ends of at least two of the separation channels.

30. A separation device, comprising:

a substrate;

a plurality of separation channels formed in said substrate, each of said separation channels having an interior bounded by a side wall;

a plurality of fluid interface ports formed in the side walls of said separation channels to provide access to the interior of the separation channel, wherein a separation medium disposed in the interior of the separation channel forms a virtual wall at the fluid interface port and wherein each separation channel of the plurality of separation channels includes at least one dedicated fluid interface port; and

an anode reservoir multiplexed to two or more of the plurality of separation channels.

31. The separation device of claim 30, wherein the fluid interface port has a diameter between about 25 μ

m and about 125 μ

m.

32. A separation device, comprising:

a substrate;

a plurality of separation channels formed in said substrate, each of said separation channels having an interior bounded by a side wall;

a plurality of fluid interface ports formed in the side walls of said separation channels to provide access to the interior of the separation channel, wherein a separation medium disposed in the interior of the separation channel forms a virtual wall at the fluid interface port and wherein each separation channel of the plurality of separation channels includes at least one dedicated fluid interface port; and

a cathode reservoir multiplexed to two or more of the plurality of separation channels.

33. The separation device of claim 32, wherein the fluid interface port has a diameter between about 25 μ

m and about 125 μ

m.

34. The device of claim 32, further comprising an array of electrodes coupled to the substrate.

35. The device of claim 32, wherein said plurality of fluid interface ports are regularly spaced in said substrate and adapted to engage a parallel loading device.

36. The device of claim 35, wherein the parallel loading device comprises a multi-headed pipetter.

37. The separation device of claim 36, wherein the parallel loading device comprises a pin for carrying and introducing the droplet of a liquid sample to the fluid interface port by contacting the virtual wall.

38. The separation device of claim 32, wherein the separation channels are disposed in a radial pattern on the separation device.

39. A method for injecting a liquid sample through a separation device, comprising the steps of:

connecting a cathode reservoir to respective first ends of two or more separation channels;

connecting an anode reservoir to respective second ends of two or more of said separation channels;

forming a droplet from the liquid sample;

directing the droplet to a virtual wall formed by a separation medium in a fluid interface port formed in a side wall of a separation channel; and

applying a voltage to the fluid interface port to draw the sample into the separation channel.

40. A method of forming a separation device for separating a sample into different components, comprising the steps of:

forming a plurality of separation channels in said separation device, each of said separation channels defined by an interior bounded by a side wall;

forming a plurality of fluid interface ports in the side walls of said separation channels to provide access to the interior of the separation channels, wherein each fluid interface port forms a virtual wall when the separation channels are filled with a separation medium; and

connecting an anode reservoir to two or more of the plurality of separation channels.

41. The method of claim 40, wherein the step of forming a plurality of fluid interface ports comprises removing portions of said side walls to define an aperture having a diameter between about 25 μ

m and about 125 μ

m.

42. The method of claim 40, wherein the separation channels are disposed in a radial pattern on the separation device.

43. A method of forming a separation device for separating a sample into different components, comprising the steps of:

forming a plurality of separation channels in said separation device, each of said separation channels defined by an interior bounded by a side wall;

forming a plurality of fluid interface ports in the side walls of said separation channels to provide access to the interior of the separation channels, wherein each fluid interface port forms a virtual wall when the separation channels are filled with a separation medium; and

connecting a cathode reservoir to two or more of the plurality of separation channels.

44. The method of claim 43, wherein the step of forming a plurality of fluid interface ports comprises removing portions of said side walls to define an aperture having a diameter between about 25 μ

m and about 125 μ

m

45. The method of claim 43, wherein the separation channels are disposed in a radial pattern on the separation device.