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Dual bead assays including optical biodiscs and methods relating thereto

  • US 20030003464A1
  • Filed: 11/27/2001
  • Published: 01/02/2003
  • Est. Priority Date: 11/27/2000
  • Status: Abandoned Application
First Claim
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1. A method for identifying whether a target agent is present in a biological sample comprising:

  • mixing capture beads, each having at least one transport probe affixed thereto, reporter beads, each having at least one signal probe affixed thereto, and a biological sample, under binding conditions so as to permit formation of a dual bead complex if the target agent is present in the sample, the reporter bead and capture bead each being bound to the target agent;

    isolating the dual bead complex from the mixture to obtain an isolate;

    exposing the isolate to a capture field on a disc, the capture field having a capture agent that binds to the dual bead complex; and

    detecting the presence of the dual bead complex in the disc to indicate that the target agent is present in the sample.

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