Porous inorganic substrate for high-density arrays
First Claim
1. A substrate for attaching an array of biological or chemical analytes, said substrate comprises:
- a) a porous, predominantly inorganic layer adhered to a flat, rigid, non-porous, inorganic understructure;
b) said porous inorganic layer characterized as having a plurality of interconnected voids of a predetermined mean size of not less than about 0.1 μ
m dispersed therethrough, and having void channels that extend through to a top surface of said porous inorganic layer; and
said voids are defined by a network of inorganic material having a predetermined mean particle size of not less than about 0.1 μ
m; and
c) said inorganic material and contents of said voids exhibit a high contrast in their indices of refraction relative to each other.
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Accused Products
Abstract
A porous inorganic substrate and method of fabricating such substrate for attaching an array of biological or chemical molecules to be used in a high-density microarray device. The substantially planar substrate comprises a porous inorganic layer adhered to a flat, rigid, non-porous, inorganic understructure having a coefficient of thermal expansion compatible with that of the porous inorganic layer. The porous inorganic layer is characterized as having dispersed throughout it a plurality of interconnecting voids as defined by a network of contiguous inorganic material, each of a predetermined mean size. The continuous inorganic material and contents of the voids exhibit a high contrast in their indices of refraction relative to each other. The substrate further comprises a uniform coating of a binding agent over at least a part of the surface area of the voids and the top surface of the porous inorganic layer.
19 Citations
58 Claims
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1. A substrate for attaching an array of biological or chemical analytes, said substrate comprises:
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a) a porous, predominantly inorganic layer adhered to a flat, rigid, non-porous, inorganic understructure;
b) said porous inorganic layer characterized as having a plurality of interconnected voids of a predetermined mean size of not less than about 0.1 μ
m dispersed therethrough, and having void channels that extend through to a top surface of said porous inorganic layer; and
said voids are defined by a network of inorganic material having a predetermined mean particle size of not less than about 0.1 μ
m; and
c) said inorganic material and contents of said voids exhibit a high contrast in their indices of refraction relative to each other. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A device for performing multiple assays, said device includes:
- a planar substrate comprising a porous inorganic layer adhered to a flat, rigid, non-porous, impermeable, inorganic understructure having a coefficient of thermal expansion compatible with that of said porous inorganic layer;
said porous inorganic layer characterized as having a plurality of interconnected voids of a predetermined mean size of not less than about 0.1 μ
m and not greater than about 15 μ
m and having void channels that extend through to a top surface of said porous inorganic layer; and
said voids are defined by a network of contiguous inorganic material having a predetermined mean size of not less than about 0.1 μ
m and not greater than about 15 μ
m;
said contiguous inorganic material and contents of said voids exhibit a high contrast in their indices of refraction relative to each other; and
having a coating of a binding agent over at least a portion of the surface area of said voids and said top surface of said porous inorganic layer. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
- a planar substrate comprising a porous inorganic layer adhered to a flat, rigid, non-porous, impermeable, inorganic understructure having a coefficient of thermal expansion compatible with that of said porous inorganic layer;
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37. A method of forming a substrate for attaching an array of biological or chemical analytes, said method comprises:
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a) providing a flat, rigid, non-porous, impermeable, inorganic understructure;
b) applying a porous, predominantly inorganic layer to a top surface of said inorganic understructure;
wherein said porous inorganic layer is formed by an process that comprises;
1) applying a layer of individual particles of an inorganic material to a top surface of said inorganic understructure, said particles having a predetermined mean size of not less than about 0.1 μ
m;
2) forming a network of inorganic material from said individual particles to create a plurality of interconnected voids of a predetermined mean size of not less than about 0.1 μ
m dispersed through-out said porous inorganic layer, and having void channels that extend through to a top surface of said porous inorganic layer;
c) configuring said inorganic material and contents of said voids to exhibit a high contrast in their indices of refraction relative to each other;
d) preparing said top surface of said porous inorganic layer for binding biological or chemical analytes.
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Specification