Novel promoter of gene expression
First Claim
1. An isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its −
- 10 region.
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Abstract
The invention provides an isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its 10 region together with DNA constructs, expression vectors and transformed cells containing such molecules. Also provided by the invention is a method for assaying promoter activity, comprising expressing in an antibiotic-susceptible (i.e. sensitive) host cell, an antibiotic resistance gene under the control of the promoter to be assayed, and assessing the growth of said cell in the presence of said antibiotic.
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Citations
19 Claims
- 1. An isolated DNA molecule comprising a promoter sequence, said promoter sequence being a Pm or a Pu promoter of a TOL plasmid, said promoter sequence having sequence modifications in its −
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19. A method for assaying promoter activity, said method comprising expressing in an antibiotic-susceptible (i.e. sensitive) host cell, an antibiotic resistance gene under the control of the promoter to be assayed, and assessing the growth of said cell in the presence of said antibiotic.
Specification