Carotenoid production from a single carbon substrate
First Claim
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1. A method for the production of a carotenoid compound comprising:
- (a) providing a transformed C1 metabolizing host cell comprising;
(i) suitable levels of isopentenyl pyrophosphate; and
(ii) at least one isolated nucleic acid molecule encoding an enzyme in the carotenoid biosynthetic pathway under the control of suitable regulatory sequences;
(b) contacting the host cell of step (a) under suitable growth conditions with an effective amount of a C1 carbon substrate whereby an carotenoid compound is produced.
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Abstract
A method for the production of carotenoid compounds is disclosed. The method relies on the use of microorganisms which metabolize single carbon substrates for the production of carotenoid compounds in high yields.
53 Citations
50 Claims
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1. A method for the production of a carotenoid compound comprising:
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(a) providing a transformed C1 metabolizing host cell comprising;
(i) suitable levels of isopentenyl pyrophosphate; and
(ii) at least one isolated nucleic acid molecule encoding an enzyme in the carotenoid biosynthetic pathway under the control of suitable regulatory sequences;
(b) contacting the host cell of step (a) under suitable growth conditions with an effective amount of a C1 carbon substrate whereby an carotenoid compound is produced. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 50)
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49. A method for the over-production of carotenoid production in a transformed C1 metabolizing host comprising:
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(a) providing a transformed Cl metabolizing host cell comprising;
(i) suitable levels of isopentenyl pyrophosphate; and
(ii) at least one isolated nucleic acid molecule encoding an enzyme in the carotenoid biosynthetic pathway under the control of suitable regulatory sequences; and
(iii) either;
1) multiple copies of at least one gene encoding an enzyme selected from the group consisting of D-1-deoxyxylulose-5-phosphate synthase (Dxs), D-1-deoxyxylulose-5-phosphate reductoisomerase (Dxr), 2C-methyl-d-erythritol cytidylyltransferase (IspD), 4-diphosphocytidyl-2-C-methylerythritol kinase (IspE), 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (IspF), CTP synthase (PyrG) lytB and gcpE;
or2) at least one gene encoding an enzyme selected from the group consisting of D-1-deoxyxylulose-5-phosphate synthase (Dxs), D-1 -deoxyxylulose-5-phosphate reductoisomerase (Dxr), 2C-methyl-d-erythritol cytidylyltransferase (IspD), 4-diphosphocytidyl-2-C-methylerythritol kinase (IspE), 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (IspF), CTP synthase (PyrG), lytB and gcpE operably linked to a strong promoter. (b) contacting the host cell of step (a) under suitable growth conditions with an effective amount of a C1 carbon substrate whereby a carotenoid compound is over-produced.
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Specification