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Novel cell culture supports with particular properties, and production thereof

  • US 20030003554A1
  • Filed: 04/29/2002
  • Published: 01/02/2003
  • Est. Priority Date: 10/29/1999
  • Status: Abandoned Application
First Claim
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1. A process for continuously producing two-dimensional (2D) supports endowed with particular properties for anchorage-dependent cell culture (ADC), comprising at least the following steps in succession:

  • continuously producing rolls of polymer film with a thickness of 35 μ

    or less and with a density in the range 0.9 to 1.25 g/cm3 from granules of a given polymer;

    activating said polymer film using any means for generating reactive groups, in particular radicals and/or peroxide, hydroperoxide or amine functions;

    producing covalent grafts between the activated polymer film and a polymer, a copolymer or a macromolecule of interest the property of which is desired, said grafting being achieved by immersing the film in a solution of monomer, copolymerisation being initiated by free radicals created by activation under β

    irradiation, the immersion time being directly correlated to the desired thickness of the polymer grafted onto the film;

    if necessary, washing to eliminate monomers that are not consumed and not fixed to the film, followed by drying the grafted film;

    cutting the grafted polymer film by continuous punching, the size of the punches being selected as a function of the desired size of the 2D supports.

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