Multipotent stem cells from peripheral tissues and uses thereof
First Claim
Patent Images
1. A cellular composition comprising a purified population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self renewing, are positive for nestin and fibronectin protein, and differentiate into both neuronal and non-neuronal cell types.
1 Assignment
0 Petitions
Accused Products
Abstract
This invention relates to multipotent stem cells, purified from the peripheral tissue of mammals, and capable of differentiating into neural and non-neural cell types. These stem cells provide an accessible source for autologous transplantation into CNS, PNS, and other damaged tissues.
-
Citations
73 Claims
- 1. A cellular composition comprising a purified population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self renewing, are positive for nestin and fibronectin protein, and differentiate into both neuronal and non-neuronal cell types.
-
2. A cellular composition comprising multipotent mammalian cells with fewer than 30 percent lineage committed cells, wherein said multipotent cells form non-adherent clusters in culture, are self renewing, are positive for nestin and fibronectin protein, and differentiate into ectodermal and mesodermal cell types.
-
3. A cellular composition comprising multipotent cells prepared by the method comprising:
-
(a) culturing a dissociated sample of epithelial tissue;
(b) isolating, from the culture, non-adherent cells characterized by the following;
are positive for nestin and fibronectin protein, are self renewing, and differentiate into ectodermal and mesodermal cell types. - View Dependent Claims (18, 20, 21, 22, 25, 26, 30)
-
-
4. A cellular composition comprising multipotent cells prepared by the method comprising:
-
(a) separating the dermal and epidermal layers of epithelial tissue;
(b) culturing a dissociated sample of the dermal layer of said epithelial tissue;
(c) isolating, from the culture, non-adherent cells characterized by the following;
are self-renewing, and differentiate into ectodermal and mesodermal cell types.
-
-
5. A cellular composition comprising a population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self-renewing, are positive for nestin and fibronectin protein, differentiate into both neuronal and non-neuronal cell types, and can proliferate in culture in the absence of exogenous EGF.
-
6. A cellular composition comprising a population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self-renewing, differentiate into both neuronal and non-neuronal cell types, and can proliferate in culture in the absence of exogenous LIF.
-
7. A cellular composition comprising a population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self-renewing, are positive for nestin and fibronectin protein, are negative for vimentin and cytokeratin protein, and differentiate into both neuronal and non-neuronal cell types.
-
8. A cellular composition comprising a population of multipotent mammalian cells, which multipotent cells form non-adherent clusters in culture, are self-renewing, are positive for nestin and fibronectin protein, are negative for vimentin, cytokeratin, and p75 protein, and differentiate into both neuronal and non-neuronal cell types.
-
50. A method for preparing stem cell preparations, comprising:
-
(a) obtaining an epithelial tissue sample;
(b) culturing cells dissociated from said tissue sample;
(c) isolating from the culture multipotent cells characterized by the following;
form non-adherent clusters in culture;
are self renewing;
express nestin and fibronectin; and
differentiate into ectodermal and mesodermal cell types.
-
-
51. A method for preparing stem cell preparations, comprising:
-
(a) obtaining an epithelial tissue sample;
(b) separating the dermal and epidermal layers of said tissue sample;
(c) culturing cells dissociated from the dermal layer of said tissue sample;
(d) isolating from the culture multipotent cells characterized by the following;
form non-adherent clusters in culture;
are self renewing; and
differentiate into ectodermal and mesodermal cell types.
-
-
52. A method for preparing stem cell preparations, comprising:
-
(a) obtaining an epithelial tissue sample from a patient;
(b) culturing cells dissociated from said tissue sample;
(c) isolating from the culture multipotent cells characterized by the following;
form non-adherent clusters in culture;
are self renewing;
express nestin and fibronectin; and
differentiate into ectodermal and mesodermal cell types; and
(d) preserving and storing the multipotent cells for later retrieval. - View Dependent Claims (55, 56, 59, 60, 61)
-
-
53. A method for preparing cell preparations, comprising:
-
(a) obtaining an epithelial tissue sample from a patient;
(b) culturing cells dissociated from said tissue sample under conditions wherein multipotent cells are expanded, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing;
express nestin and fibronectin; and
differentiate into ectodermal and mesodermal cell types;
(c) differentiating the multipotent cells into one or more lineage committed cell types; and
(d) preserving and storing the differentiated cells for later retrieval.
-
-
54. A method for preparing cell preparations, comprising:
-
(a) obtaining an epithelial tissue sample from a patient;
(b) culturing cells dissociated from said tissue sample under conditions wherein multipotent cells are expanded, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing;
express nestin and fibronectin; and
differentiate into ectodermal and mesodermal cell types;
(c) differentiating the multipotent cells into one or more lineage committed cell types, wherein the conditions for differentiating the multipotent cells include modulating the plating conditions; and
(d) preserving and storing the differentiated cells for later retrieval.
-
-
57. A method for conducting a regenerative medicine business, comprising:
-
(a) a service for accepting and logging in epithelial tissue samples from a client;
(b) a cell culture system for culturing cells dissociated from said tissue sample, which system provides conditions suitable for expanding multipotent cells in said sample, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing; and
differentiate into ectodermal and mesodermal cell types;
(c) a cell preservation system for preserving said multipotent cells for later retrieval on behalf of said client or other third party.
-
-
58. A method for conducting a regenerative medicine business, comprising:
-
(a) a service for accepting and logging in epithelial tissue samples from a client;
(b) a cell culture system for culturing cells dissociated from said tissue sample, which system provides conditions suitable for expanding multipotent cells in said sample, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing; and
differentiate into ectodermal and mesodermal cell types;
(c) a cell differentiation system for differentiating said multipotent cells into one or more lineage committed cell types (d) a cell preservation system for preserving said lineage committed cells for later retrieval on behalf of said client or other third party.
-
-
68. A cellular composition of adult stem cells which (i) will proliferate in an in vitro culture, (ii) maintains the potential to differentiate to derivatives of endoderm, mesoderm, and ectoderm tissues throughout the culture, and (iii) is inhibited from differentiation when cultured under proliferative conditions.
-
69. A cellular composition of adult stem cells which stem cells which (i) will proliferate in an in vitro culture for over one year, (ii) maintains a karyotype in which the chromosomes are euploid and not altered through prolonged culture, (iii) maintains the potential to differentiate to derivatives of endoderm, mesoderm, and ectoderm tissues throughout the culture, and (iv) is inhibited from differentiation when cultured under proliferative conditions.
-
70. A method for conducting a regenerative medicine business, comprising:
-
(a) a service for accepting and logging in epithelial tissue samples from a client;
(b) a cell culture system for culturing cells dissociated from said tissue sample, which system provides conditions suitable for expanding multipotent cells in said sample, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing; and
differentiate into ectodermal, mesodermal, and endodermal cell types;
(c) a cell preservation system for preserving said multipotent cells for later retrieval on behalf of said client or other third party. - View Dependent Claims (72, 73)
-
-
71. A method for conducting a regenerative medicine business, comprising:
-
(a) a service for accepting and logging in epithelial tissue samples from a client;
(b) a cell culture system for culturing cells dissociated from said tissue sample, which system provides conditions suitable for expanding multipotent cells in said sample, which multipotent cells are characterized by the following;
form non-adherent clusters in culture;
are self renewing; and
differentiate into ectodermal, mesodermal, and endodermal cell types;
(c) a cell differentiation system for differentiating said multipotent cells into one or more lineage committed cell types (d) a cell preservation system for preserving said lineage committed cells for later retrieval on behalf of said client or other third party.
-
Specification