Apparatus and method of monitoring an analyte

• US 20030003593A1
• Filed: 08/05/2002
• Published: 01/02/2003
• Est. Priority Date: 01/31/2001
• Status: Active Grant

• Current Assignee: Grouptek Incorporated
• Original Assignee: Grouptek Incorporated
• Inventors: Wallach, Donald F.H.
• Granted Patent: US 6,576,474 B2
• Time in Patent Office: Days
• US Class Current: 436/170
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

A detector for monitoring an analyte includes an analyte-sensing composition. The analyte-sensing composition has a visible color intensity or emission intensity (e.g., fluorescence intensity) that changes as the analyte concentration contacting the detector changes. The intensity changes can be visible to the human eye, or identified by an instrument. The analyte can include carbon dioxide, a volatile amine or a volatile carboxylic acid.

Claims

1. A method of detecting an analyte comprising:

contacting a detector with a food product, body fluid or tissue, the detector including;

a carrier;

an analyte-sensing composition including an indicator dye, a buffer, and an osmotic control agent, the analyte-sensing composition being contained by the carrier;

a hydrophobic barrier on one surface of the carrier, the hydrophobic barrier being arranged to contact the food product, body fluid or tissue, and

an outer barrier covering a surface of the carrier opposite the hydrophobic barrier; and

monitoring the detector for a change in visible color intensity or emission intensity from the indicator dye which indicates the presence of the analyte.

2. The method of claim 1, wherein monitoring includes detecting the change with an instrument.

3. The method of claim 1, wherein the analyte includes carbon dioxide, a volatile amine or a volatile carboxylic acid.

4. The method of claim 1, wherein the detector is a portion of a food package or a hygiene product.

5. The method of claim 1, wherein the presence of the analyte changes the pH within the carrier.

6. The method of claim 1, further comprising removing the detector from a sealed package prior to contacting the food product, body fluid or tissue.

7. A method of manufacturing a detector comprising:

depositing a solution containing a solvent and an analyte-sensing composition on a carrier, the analyte-sensing composition including an indicator dye, a buffer, and an osmotic control agent;

removing the solvent;

forming a hydrophobic barrier on a surface of the carrier; and

placing an outer barrier on a surface of the carrier opposite the hydrophobic barrier.