Low intensity light therapy for the manipulation of fibroblast, and fibroblast-derived mammalian cells and collagen

• US 20030004556A1
• Filed: 04/11/2002
• Published: 01/02/2003
• Est. Priority Date: 11/30/1998
• Status: Active Grant

• Current Assignee: L'Oreal SA
• Original Assignee: Light BioScience LLC
• Inventors: McDaniel, David H.
• Granted Patent: US 6,676,655 B2
• Time in Patent Office: Days
• US Class Current: 607/88
• CPC Class Codes: A61B 18/203 applying laser energy to the outside of the body
• CPC Class Codes: A61B 2018/00023 closed, i.e. without wound contact by the fluid
• CPC Class Codes: A61B 2018/00452 Skin
• CPC Class Codes: A61B 2018/0047 Upper parts of the skin, e.g. skin peeling or treatment of wrinkles
• CPC Class Codes: A61B 2018/00702 Power or energy
• CPC Class Codes: A61B 2018/00791 Temperature
• CPC Class Codes: A61B 2090/395 with marking agent for marking skin or other tissue
• CPC Class Codes: A61K 2800/58 Metal complex; Coordination compounds
• CPC Class Codes: A61K 2800/782 Enzyme inhibitors; Enzyme antagonists
• CPC Class Codes: A61K 2800/81 Preparation or application process involves irradiation
• CPC Class Codes: A61K 41/0057 Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
• CPC Class Codes: A61K 41/0071 PDT with porphyrins having exactly 20 ring atoms, i.e. based on the non-expanded tetrapyrrolic ring system, e.g. bacteriochlorin, chlorin-e6, or phthalocyanines
• CPC Class Codes: A61K 8/19 containing inorganic ingredients
• CPC Class Codes: A61K 8/31 Hydrocarbons
• CPC Class Codes: A61K 8/347 Phenols
• CPC Class Codes: A61K 8/35 Ketones, e.g. benzophenone
• CPC Class Codes: A61K 8/361 Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
• CPC Class Codes: A61K 8/365 Hydroxycarboxylic acids; Ketocarboxylic acids
• CPC Class Codes: A61K 8/368 with carboxyl groups directly bound to carbon atoms of aromatic rings
• CPC Class Codes: A61K 8/49 containing heterocyclic compounds
• CPC Class Codes: A61K 8/494 : with more than one nitrogen as the only hetero atom
• CPC Class Codes: A61K 8/4953 : containing pyrimidine ring derivatives, e.g. minoxidil
• CPC Class Codes: A61K 8/498 : having 6-membered rings or their condensed derivatives, e.g. coumarin
• CPC Class Codes: A61K 8/67 : Vitamins
• CPC Class Codes: A61K 8/671 : Vitamin A; Derivatives thereof, e.g. ester of vitamin A acid, ester of retinol, retinol, retinal
• CPC Class Codes: A61K 8/676 : Ascorbic acid, i.e. vitamin C
• CPC Class Codes: A61K 8/678 : Tocopherol, i.e. vitamin E
• CPC Class Codes: A61K 8/9706 : Algae
• CPC Class Codes: A61K 8/9789 : Magnoliopsida [dicotyledons]
• CPC Class Codes: A61N 2005/0652 : Arrays of diodes
• CPC Class Codes: A61N 5/0617 : Hair treatment
• CPC Class Codes: A61N 5/062 : Photodynamic therapy, i.e. excitation of an agent
• CPC Class Codes: A61Q 19/08 : Anti-ageing preparations

Abstract

Disclosed is a method for treating various dermatalogical conditions using electromagnetic radiation. Particularly preferred are narrowband, multichromatic electromagnetic radiation emitters having a dominant emissive wavelength corresponding to the peak absorption wavelength of the mammalian tissue targetted for treatment. Topical compositions are disclosed for pretreating the targetted tissue to alter the peak absorption wavelength of the tissue.

Claims

1. A method for the manipulation of collagen, fibroblast, and fibroblast-derived cell levels in mammalian tissue comprising:

exposing said tissue to a plurality of pulses from at least one source of narrowband, multichromatic electromagnetic radiation having a dominant emissive wavelength of from about 300 nm to about 1600 nm, wherein said pulses have a duration of from about 0.1 femtoseconds to about 100 seconds, the interpulse delay between said pulses is from about 0.1 to about 1000 milliseconds, and the energy fluence received by said tissue is less than about 10 joule per square centimeter.

2. The method of claim 1 wherein said source of narrowband, multichromatic electromagnetic radiation is selected from a light emitting diode, a laser, a fluorescent light source, an organic light emitting diode, a light emitting polymer, a xenon arc lamp, a metal halide lamp, a filamentous light source, an intense pulsed light source, a sulfur lamp, and combinations thereof, and said dominant emissive wavelength is from about 400 nm to about 1600 nm.

3. The method of claim 2 wherein said source of narrowband, multichromatic electromagnetic radiation further comprises a filter element for reducing the intensity of infrared radiation received by said tissue

4. The method according to claim 1 wherein the energy fluence received at said tissue is 1 J/cm² or less.

5. The method according to claim 1 wherein the energy fluence received at said tissue is greater than 1 J/cm² and further comprises cooling said tissue.

6. The method according to claim 4 wherein said energy fluence received at said tissue is from about from about 1×

10<sup>−

6</sup> J/cm² to 1 j/cm².

7. The method of claim 6 wherein said energy fluence received at said tissue is from about from about 1×

10<sup>−

3</sup> J/cm to about 0.1 J/cm².

8. The method of claim 1 wherein said pulse length is from about 1 nanosecond to about 1 second.

9. The method of claim 8 wherein said pulse length is from about 5 nanoseconds to about 100 milliseconds.

10. The method of claim 1 wherein said source of electromagnetic radiation is filtered to reduce the energy fluence of infrared radiation.

11. The method of claim 1 wherein said source of electromagnetic radiation is filtered to reduce the perception by said tissue of radiation having a wavelength greater than about 700 nm.

12. The method of claim 1 further comprising applying a topical composition to said tissue, prior to exposing said tissue.

14. The method of claim 13 wherein said source of narrowband, multichromatic electromagnetic radiation is selected from a light emitting diode, a laser, a fluorescent light source, an organic light emitting diode, a light emitting polymer, a xenon arc lamp, a metal halide lamp, a filamentous light source, a sulfur lamp, and combinations thereof, and said dominant emissive wavelength is from about 400 nm to about 1600 nm.

15. The method of claim 14 wherein said source of narrowband, multichromatic electromagnetic radiation further comprises a filter element for reducing the intensity of infrared radiation received by said tissue

16. The method according to claim 13 wherein the energy fluence received at said tissue is 1 J/cm² or less.

17. The method according to claim 13 wherein the energy fluence received at said tissue is greater than 1 J/cm² and further comprises cooling said tissue.

18. The method according to claim 16 wherein said energy fluence received at said tissue is from about from about 1×

10<sup>−

6</sup> J/cm² to 1 J/cm².

19. The method of claim 18 wherein said energy fluence received at said tissue is from about from about 1×

10<sup>−

3</sup> J/cm² to about 0.1 J/cm².

20. The method of claim 13 wherein said source of electromagnetic radiation is filtered to reduce the energy fluence of infrared radiation.

21. The method of claim 13 wherein said source of electromagnetic radiation is filtered to reduce the perception by said tissue of radiation having a wavelength greater than about 700 nm.

22. The method of claim 13 further comprising applying a topical composition to said tissue, prior to exposing said tissue.

23. The method of claim 22 wherein said topical composition is selected from the group consisting of naturally occurring chlorophyll-containing compounds, carotenoid-containing compounds, phyocobilin compounds, indocyanine green, methylene blue, rose Bengal, Vitamin C, Vitamin E, Vitamin D, Vitamin A, Vitamin K, Vitamin F, Retin A (Tretinoin), Adapalene, Retinol, Hydroquinone, Kojic acid, a growth factor, echinacea, an antibiotic, an antifungal, an antiviral, a bleaching agent, an alpha hydroxy acid, a beta hydroxy acid, salicylic acid, antioxidant triad compound, a seaweed derivative, a salt water derivative, algae, an antioxidant, a phytoanthocyanin, a phytonutrient, plankton, a botanical product, a herbaceous product, a hormone, an enzyme, a mineral, a cofactor, an antiaging substance, insulin, minoxidil, lycopene, a natural or synthetic melanin, a metalloproteinase inhibitor, proline, hydroxyproline, an anesthetic, chlorophyll, bacteriochlorophyll, copper chlorophyllin, chloroplasts, carotenoids, phycobilin, rhodopsin, anthocyanin, inhibitors of omithine decarboxylase, inhibitors of vascular endothelial growth factor (VEGF), inhibitors of phospholipase A2, inhibitors of S-adenosylmethionine, licorice, licochalone A, genestein, soy isoflavones, phtyoestrogens, derivative, analogs, homologs, and subcomponents thereof, and derivatives, subcomponents, immunological complexes and antibodies of said target tissue, and synthetic and natural analogs thereof, and combinations thereof.

24. The method of claim 1 further comprising cooling said tissue to maintain a temperature of said tissue below the threshold for thermal injury.

25. The method of claim 13 further comprising cooling said tissue to maintain a temperature of said tissue below the threshold for thermal injury.

26. The method of claim 1 further comprising maintaining the temperature of said tissue at or below 38°

C.

27. The method of claim 13 further comprising the step of maintaining the temperature of said tissue at or below 38°

C.

13. The method of claim 12 wherein said topical composition is selected from the group consisting of naturally occurring chlorophyll-containing compounds, carotenoid-containing compounds, phyocobilin compounds, indocyanine green, methylene blue, rose Bengal, Vitamin C, Vitamin E, Vitamin D, Vitamin A, Vitamin K, Vitamin F, Retin A (Tretinoin), Adapalene, Retinol, Hydroquinone, Kojic acid, a growth factor, echinacea, an antibiotic, an antifungal, an antiviral, a bleaching agent, an alpha hydroxy acid, a beta hydroxy acid, salicylic acid, antioxidant triad compound, a seaweed derivative, a salt water derivative, algae, an antioxidant, a phytoanthocyanin, a phytonutrient, plankton, a botanical product, a herbaceous product, a hormone, an enzyme, a mineral, a cofactor, an antiaging substance, insulin, minoxidil, lycopene, a natural or synthetic melanin, a metalloproteinase inhibitor, proline, hydroxyproline, an anesthetic, chlorophyll, bacteriochlorophyll, copper chlorophyllin, chloroplasts, carotenoids, phycobilin, rhodopsin, anthocyanin, inhibitors of omithine decarboxylase, inhibitors of vascular endothelial growth factor (VEGF), inhibitors of phospholipase A2, inhibitors of S-adenosylmethionine, licorice, licochalone A, genestein, soy isoflavones, phtyoestrogens, derivative, analogs, homologs, and subcomponents thereof, and derivatives, subcomponents, immunological complexes and antibodies of said target tissue, and synthetic and natural analogs thereof, and combinations thereof.

13-1. A method for the manipulation of collagen, fibroblast, and fibroblast-derived cell levels in mammalian tissue comprising:

exposing said tissue to at least one source of narrowband, multichromatic electromagnetic radiation having a dominant emissive wavelength of from about 300 nm to about 1600 nm for a period of time of from about 10 seconds to about 24 hours, wherein the energy fluence received by said tissue is less than about 10 J/cm².