Data processing of the maize prolifera genetic sequence

• US 20030005483A1
• Filed: 05/09/2002
• Published: 01/02/2003
• Est. Priority Date: 07/09/1999
• Status: Active Grant

• Current Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Original Assignee: Pioneer Hi Bred International Inc (Corteva, Inc.)
• Inventors: Simmons, Carl R., Nadimpalli, Ramgopal
• Granted Patent: US 6,879,916 B2
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: G16B 30/00 ICT specially adapted for sequence analysis involving nucleotides or amino acids
• CPC Class Codes: G16B 30/10 Sequence alignment; Homology search
• CPC Class Codes: Y10S 707/99931 Database or file accessing

Abstract

The invention provides isolated prolifera nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering prolifera levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Claims

1. An isolated nucleic acid comprising a member selected from the group consisting of:

(a) a polynucleotide having at least 85% sequence identity, as determined by the BLAST 2.0 algorithm under default parameters, to a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NO;

2;

(b) a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NO;

2;

(c) a polynucleotide amplified from a Zea mays nucleic acid library using primers which selectively hybridize, under stringent hybridization conditions, to loci within a polynucleotide selected from the group consisting of SEQ ID NO;

1;

(d) a polynucleotide which selectively hybridizes, under stringent hybridization conditions and a wash in 2×

SSC at 50°

C., to a polynucleotide selected from the group consisting of SEQ ID NO;

1;

(e) a polynucleotide selected from the group consisting of SEQ ID NO;

1;

(f) a polynucleotide which is complementary to a polynucleotide of (a), (b), (c), (d), or (e); and

(g) a polynucleotide comprising at least 25 contiguous nucleotides from a polynucleotide of (a), (b), (c), (d), (e), or (f).

2. A recombinant expression cassette, comprising a member of claim 1 operably linked, in sense or anti-sense orientation, to a promoter.

3. A host cell comprising the recombinant expression cassette of claim 2.

4. A transgenic plant comprising a recombinant expression cassette of claim 2.

5. The transgenic plant of claim 4, wherein said plant is a monocot.

6. The transgenic plant of claim 4, wherein said plant is selected from the group consisting of:

maize, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, and millet.

7. A transgenic seed from the transgenic plant of claim 4.

8. A method of modulating the level of prolifera in a plant, comprising:

(a) introducing into a plant cell a recombinant expression cassette comprising a prolifera polynucleotide of claim 1 operably linked to a promoter;

(b) culturing the plant cell under plant cell growing conditions; and

(c) inducing expression of said polynucleotide for a time sufficient to modulate the level of prolifera in said plant.

9. The method of claim 8, wherein the plant is maize.

10. An isolated protein comprising a member selected from the group consisting of:

(a) polypeptide of at least 20 contiguous amino acids from a polypeptide selected from the group consisting of SEQ ID NO;

2;

(b) a polypeptide selected from the group consisting of SEQ ID NO;

2;

(c) a polypeptide having at least 80% sequence identity to, and having at least one linear epitope in common with, a polypeptide selected from the group consisting of SEQ ID NO;

2, wherein said sequence identity is determined using BLAST 2.0 under default parameters; and

,

(d) at least one polypeptide encoded by a member of claim 1.

11. A data processing system, comprising:

data representing at least one genetic sequence;

a genetic identification, analysis, or modeling computer program designed to govern the processing of such data;

a data processor having an output for storing or displaying data processing results, said data processor containing said data and said program and executing instructions according to said program to process said data or a contiguous subsequence thereof; and

wherein said genetic sequence is;

(i) at least 90% sequence identical to a polynucleotide sequence of SEQ ID NO;

1, or (ii) at least 95% sequence identical to a polypeptide sequence of SEQ ID NO;

2, and wherein sequence identity is determined by the GAP algorithm under default parameters.

12. The data processing system of claim 11, wherein said genetic sequence is a contiguous subsegment of a gene or protein sequence contained in said data processor.

13. The data processing system of claim 12, wherein said gene or protein sequence is a genomic DNA sequence, a full-length cDNA sequence, or a polypeptide sequence.

14. The data processing system of claim 11, wherein said data processing system is a distributed system having its input and output portions separated from at least some of its processing portions.

15. The data processing system of claim 14, wherein said data processing is distributed over an intranet, an internet, or both.

16. The data processing system of claim 11, wherein said program comprises at least one of:

a sequence similarity application, a protein structure application, a sequence alignment application, a translation application, a O-glycosylation prediction application, or a signal peptide prediction application.

17. The data processing system of claim 11, wherein said data processor stores said data in a memory while processing the data, and wherein successive portions of said data are copied sequentially into at least one register of said data processor where said portions are processed.

18. The data processing system of claim 12, wherein said genetic sequence is created from said gene sequence or said protein sequence at runtime.