Comparative fluorescence hybridization to nucleic acid arrays
First Claim
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1. A method for comparing copy number of nucleic acid sequences in a two or more collections of nucleic acid molecules, the method comprising:
- (a) providing a plurality of target elements bound to a solid surface, each target element comprising a target nucleic acid;
(b) contacting the target elements with;
(i) a first collection of labelled nucleic acid comprising a sequence substantially complementary to a target nucleotide sequence, and (ii) at least a second labelled nucleic acid comprising a sequence complementary to the target nucleotide sequence;
wherein the first and second labels are distinguishable from each other; and
(c) detecting the amount of binding of the first and second labelled complementary nucleic acids to the target nucleic acids.
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Abstract
The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.
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23 Claims
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1. A method for comparing copy number of nucleic acid sequences in a two or more collections of nucleic acid molecules, the method comprising:
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(a) providing a plurality of target elements bound to a solid surface, each target element comprising a target nucleic acid;
(b) contacting the target elements with;
(i) a first collection of labelled nucleic acid comprising a sequence substantially complementary to a target nucleotide sequence, and (ii) at least a second labelled nucleic acid comprising a sequence complementary to the target nucleotide sequence;
wherein the first and second labels are distinguishable from each other; and
(c) detecting the amount of binding of the first and second labelled complementary nucleic acids to the target nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19, 20, 21, 22, 23)
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17. A kit for quantifying nucleic acid sequences in a nucleic acid sample, the kit comprising:
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(a) a solid support having an array of preselected target nucleic acids bound thereto where the array has at least two members; and
(b) a container containing reference nucleic acids, where said reference nucleic acids comprise sequences that are complementary and non-complementary to at least one member of the array.
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Specification